LOCUS       sequence-99385-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal fragment of split T7 RNAP split a position 
            179. Insert is driven by PBAD. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-N
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      642..669
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gggctagcga gctctagaaa gacttactta ataacattct ttttctaata tgaacacgat
       61 taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct
      121 ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta
      181 cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctggtgaggt
      241 tgcggataac gctgccgcca agcctctcat cactacccta ctccctaaga tgattgcacg
      301 catcaacgac tggtttgagg aagtgaaagc taagcgcggc aagcgcccga cagccttcca
      361 gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc accattaaga ccactctggc
      421 ttgcctaacc agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc
      481 cattgaggac gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa
      541 aaacgttgag gaacaactca acaagcgcgt agggcacgtc tacaagtaat gtctaacaat
      601 tcgttcaagc cgacggatat cgagctggct tggatcacac tggctcacct tcgggtgggc
      661 ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt
      721 ttattatttt ggctcgtgtt gaaatcgcga tgttgtgtct aattttgaag ttaactttga
      781 ttccattc
//
