LOCUS       sequence-99387-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            Q758C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-Q758C
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(102..129)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 accaaagcca tgacaaaaac gcgtaatttc aacacgagcc aaaataataa aaaagcagga
       61 ttaataatct ggctttttat attctctctc tagtatataa acgcagaaag gcccacccga
      121 aggtgagcca gtgtgatcca agccagctcg atatccgtcg gcttgaacga attgttagac
      181 attacttgta gacgtgccct acgcgcttgt tgagttgttc ctcaacgttt ttcttgaagt
      241 gcttagcttc aaggtcacgg atacgaccga agcgagcctc gtcctcaatg gcccgaccga
      301 ttgcgcttgc tacagcctga acggttgtat tgtcagcact ggttaggcaa gccagagtgg
      361 tcttaatggt gatgtacgct acggcttccg gcttgatttc ttgcaggaac tggaaggctg
      421 tcgggcgctt gccgcgctta gctttcactt cctcaaacca gtcgttgatg cgtgcaatca
      481 tcttagggag tagggtagtg atgagaggct tggcggcagc gttatccgca acctcaccag
      541 ctttaagttg acgctcaaac atcttgcgga agcgtgcttc acccatctcg taagactcat
      601 gctcaagggc caactgttcg cgagctaaac gctcaccgta atggtcagcc agagtgttga
      661 acgggatagc agccagttcg atgtcagaga agtcgttctt agcgatgtta atcgtgttca
      721 tattagaaaa agaa
//
