LOCUS       sequence-99389-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            Q758C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-Q758C
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct
       61 gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt
      121 gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct
      181 gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt
      241 gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac
      301 gcgcttgaac ctgatgttcc tcggtcagtt ccgcttatgc cctaccatta acaccaacaa
      361 agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag
      421 ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga
      481 atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt
      541 caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt
      601 ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc
      661 ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaatc
      721 tagagtcgac ctgcaggcat gcaagcttgg ctgtt
//
