LOCUS       sequence-9939-ex        9426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pIS1 Cyclin D2 long UTR
FEATURES             Location/Qualifiers
     source          1..9426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     944..963
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1006..1024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1969
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    7381..7502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7392..7411)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7446..7465
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7635..7739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7740..7808,7809..8600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7958..7977)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8771..9359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9260..9279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
      361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
      421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
      481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
      541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
      601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
      661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
      721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
      781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
     1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
     1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
     1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
     1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
     1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
     1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
     1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
     1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
     1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
     1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
     1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
     1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
     1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
     1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
     1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
     1981 ctataccggt ctcgatatca ctactagtgt tctagaggat gccagttggg ccgaaagaga
     2041 gagacgcgtc cataatctgg tctcttcttc tttctggttg tttttgttct ttgtgtttta
     2101 gggtgaaact taaaaaaaaa attctgcccc cacctagatc atatttaaag atcttttaga
     2161 agtgagagaa aaaggtccta cgaaaacgga ctcataaaaa gcatttggtg cctatttgaa
     2221 gtacagcata agggaatccc ttgtatatgc gaacagttat tgtttgatta tgtaaaagta
     2281 atagtaaaat gcttacagga aaacctgcag agtagttaga gaatatgtat gcctgcaata
     2341 tgggaacaaa ttagaggaga cttttttttt tcatgttatg agctagcaca tacaccccct
     2401 tgtagtataa tttcaaggaa ctgtgtacgc catttatggc atgattagat tgcaaagcaa
     2461 tgaactcaag aaggaattga aataaggagg gacatgatgg ggaaggagta caaaacaatc
     2521 tctcaacatg attgaaccat ttgggatgga gaagcacctt tgctctcagc cacctgttac
     2581 taagtcagga gtgtagttgg atctctacat taatgtcctc ttgctgtcta cagtagctgc
     2641 tacctaaaaa aagatgtttt attttgccag ttggacacag gtgattggct cctgggtttc
     2701 atgttctgtg acatcctgct tcttcttcca aatgcagttc attgcagaca ccaccatatt
     2761 gctatctaat ggggaaatgt agctatgggc cataaccaaa actcacatga aacggaggca
     2821 gatggagacc aagggtggga tccagaatgg agtcttttct gttattgtat ttaaaagggt
     2881 aatgtggcct tggcatttct tcttagaaaa aaactaattt ttggtgctga ttggcatgtc
     2941 tggttcacag tttagcattg ttataaacca ttccattcga aaagcacttt gaaaaattgt
     3001 tcccgagcga tagatgggat ggtttatgca agtcatgctg aatactcctc ccctcttctc
     3061 ttttgccccc tcccttcctg cccccagtct gggttactct tcgcttctgg tatctggcgt
     3121 tctttggtac acagttctgg tgttcctacc aggactcaag agacacccct tcctgctgac
     3181 attcccatca caacattcct cagacaagcc tgtaaactaa aatctgttac cattctgatg
     3241 gcacagaagg atcttaattc ccatctctat acttctcctt tggacatgga aagaaaagtt
     3301 attgctggtg caaagataga tggctgaaca tcagggtgtg gcattttgtt cccttttccg
     3361 tttttttttt ttttattgtt gttgttaatt ttattgcaaa gttgtattca gcgtacttga
     3421 atttttcttc ctctccactt cttagaggca ttcagttagc aaagaggttg gagcaacaac
     3481 tttttttttt ttttttgcac aattgtaatt gacaggtaat gaagctattt gttaaaatat
     3541 ttgccttttt aagtaaaaaa gaaaaatcag aacagggcta tttgaagaat tattttatac
     3601 acagattctg ccttgtttca tagtatgagg gttgaagacg gaaaacaatc taagggtctc
     3661 tcattttttt aattttgttt tgttcagttt ggtttttttt tttttttgcg ctgctaagaa
     3721 gctaaagtca tccatcctta ttcacgttga cagtacctag ctgtaatgtt tcacagagtg
     3781 tgctgctatt ttataaacat ttttataata tattatttta ctgcttaaat tccaagtcct
     3841 gaagtagatg gttgagatat gagttcttcg tactggaaaa gcccttccgt agtttgtttt
     3901 cttctggtag catattcatg gttgtttttt tttttctttt ttggtttttt ggtttttttt
     3961 ttttcctctg atcacattct tcaaagacgg agtattcttt acctcaggtt tactggacaa
     4021 aatcaataac tacaaaaggc aatgattcac gcttttgttt tcataatacc tcacaaccgt
     4081 acagtttctg cttgggagcc cattcgcatg aggaatacag aagcagtgtg agcagggctg
     4141 actccctctc aggtggaagg cagggcggtc tcactcccag ggaccttttt ggtcatggag
     4201 gccatcgggc tcccagttag accctggtat cctcatcatg atggaaaaaa tacattgaac
     4261 caagggatcc tccctcccct tcaaggcaga cgttcagtac aaacatttat gcggtaggct
     4321 cagatgtcgt aatttgcact taggtaccag gtgtcaggaa acagactaaa aagaattcca
     4381 ccaggctgtt tggagatcct catcttggag ctttttcaaa agcggggctt catctgcaaa
     4441 gggccctttc atcttgaagt ttttcccctc cgtctttccc ctcccctggc atggacacct
     4501 tgtgtttagg atcatctctg caggtttcct aggtctgaat ctgcgagtag atgaacctgc
     4561 agcaagcagc gtttatggtg cttccttctc cctcctctgt ctcaaactgc gcaggcaagc
     4621 actatgcaag cccaggccct ctgctgagcg gtactaaacg gtcgggtttt caatcacact
     4681 gaattggcag gataagaaaa ataggtcaga taagtatggg atgatagttg aagggaggtg
     4741 aagaggctgc ttctctacag aggtgaaatt ccagatgagt cagtctcttg ggaagtgtgt
     4801 ttagaagggt tcaggacttt gtgagttagc atgaccctaa aattctaggg gatttctggt
     4861 gggacaatgg gtggtgaatt ttgaagtttt ggagagggaa gtggagcagc cagcaagtaa
     4921 gctagccaga gttttctcaa gagccagctt tgctcagcac actctcctgg gccccaagga
     4981 gtcccacgga atggggaaag tgggaaccct ggagttcttg ggaatcttgg agcctaaaga
     5041 gaaaccgagg tgcaaattca tttcatggtg actgaccctt gagcttaaac agaagcagca
     5101 aatgaaagaa ccggacaaat aaggaagggc acaagcctac ccgactctat ttacagtctg
     5161 taactttcca ctcttcctgt agtcccgagg cccctgggtc cttctagctt ttctctttcc
     5221 catccttggg gccttgtgtg atgatgggtg tggggctgcc gatgggaaag tcgggggttg
     5281 ttaggctttt ctgcctgctc ctgcttaaac acaagaagga atcctggatt ttgccctctc
     5341 cttagctctt agtctctttg gtaggagttt tgttccagag gagctctccc ccttggattt
     5401 gaacttgctc tttttgttgt tgttgttctt tctcttcttt ttcttacctc ccactaaagg
     5461 ggttccaaat tatcctggtc tttttctacc ttgttgtgtt tctatctcgt ctttacttcc
     5521 atctgtttgt ttttttctcc atcagtgggg gccgagttgt tcccccagcc tgccaaattt
     5581 tgatccttcc cctcttttgg ccaaatccta gggggaagaa atcctagtat gccaaaaata
     5641 tatgctaagc ataattaaac tccatgcggg tccataacag ccaagaagcc tgcaggagaa
     5701 agccaagggc agttccctcc gcagaacacc ccatgcgtgc tgagaggcga gctccttgaa
     5761 gaaggggctg ttcttccagg aggccttatt ttgaactgcc tcaggacccc actggagagc
     5821 acagcatgcc ttactactgg gtcatccttg gtctatgtgc tctgtactgg aggctctgtt
     5881 ctgcctctta tcagccaggt caggggcaca catggcttaa gtgacaaagc cagaggagaa
     5941 gacaaccctg acagcatcac gctgcatccc attgctagca ggattggcaa ctcttcagac
     6001 ggagctgcgc ttccctgcag tctagcacct ctagggcctc tccagactgt gccctgggag
     6061 ctctgggact gaaaggttaa gaacataagg caggatcaga tgactctctc caagagggca
     6121 ggggaatttt ctctccatgg gccacagggg acagggctgg gagaagaaat agacttgcac
     6181 cttatgtcat gtaaataatt gattttctag ttcaagaaga taatattggt agtgtgggaa
     6241 ttggaggtag gaaggggagg aagtctgagt aagccagttg gcttctaagc caaaaggatt
     6301 cctctttgtt tatctctgag acagtccaac cttgagaata gctttaaaag ggaaattaat
     6361 gctgagatga taaagtcccc ttaagccaac aaaccctctg tagctataga atgagtgcag
     6421 gtttctattg gtgtggactc agagcaattt acaagagctg ttcatgcagc catccatttg
     6481 tgcaaaatag ggtaagaaga ttcaagagga tatttattac ttcctcatac cacatggctt
     6541 ttgatgattc tggattctaa acaacccaga atggtcattt caggcacaac gatactacat
     6601 tcgtgtgtgt ctgcttttaa acttggctgg gctatcagac cctattctcg gctcaggttt
     6661 tgagaagcca tcagcaaatg tgtacgtgca tgctgtagct gcagcctgca tcccttcgcc
     6721 tgcagcctac tttggggaaa taaagtgcct tactgactgt agccattaca gtatccaatg
     6781 tcttttgaca ggtgcctgtc cttgaaaaac aaagtttcta tttttatttt taattggttt
     6841 agttcttaac tgctggccaa ctcttacatc cccagcaaat catcgggcca ttggattttt
     6901 tccattatgt tcatcaccct tatatcatgt acctcagatc tctctctctc tcctctctct
     6961 cagttatata gtttcttgtc ttggactttt tttttctttt ctttttcttt ttttttttgc
     7021 tttaaaacaa gtgtgatgcc atatcaagtc catgttattc tctcacagtg tactctataa
     7081 gaggtgtggg tgtctgtttg gtcaggatgt tagaaagtgc tgataagtag catgatcagt
     7141 gtatgcgaaa aggtttttag gaagtatggc aaaaatgttg tattggctat gatggtgaca
     7201 tgatatagtc agctgccttt taagaggtct tatctgttca gtgttaagtg atttaaaaaa
     7261 ataataacct gttttctgac tagtttaaag atggatttga aaatggtttt gaatgcaatt
     7321 aggttatgct atttggacaa taaactcacc ttgacctgcg gccgcttcga gcagacatga
     7381 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     7441 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     7501 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     7561 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt
     7621 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7681 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7741 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7801 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7861 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7921 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7981 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8041 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8101 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8161 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8221 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8281 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8341 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8401 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8461 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8521 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8581 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8641 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8701 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8761 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8821 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8881 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8941 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9001 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9061 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9121 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9181 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9241 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9301 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9361 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg
     9421 ctcgac
//
