LOCUS       sequence-99393-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by PBAD. The C-terminal fragment has the point 
            mutation R756K. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-R756K
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(353..380)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc
       61 ttatcatcga tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat
      121 gctactccgt caagccgtca attgtctgat tcgttaccaa cagacattgc cgtcactgcg
      181 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
      241 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaattt caacacgagc caaaataata
      301 aaaaagcagg attaataatc tggcttttta tattctctct ctagtatata aacgcagaaa
      361 ggcccacccg aaggtgagcc agtgtgatcc aagccagctc gatatccgtc ggcttgaacg
      421 aattgttaga cattacttgt agacgtgccc tacgcgcttg ttgagttgtt cctcaacgtt
      481 tttcttgaag tgcttagctt caaggtcacg gatacgaccg aagcgagcct cgtcctcaat
      541 ggcccgaccg attgcgcttg ctacagcctg aacggttgta ttgtcagcac tggttaggca
      601 agccagagtg gtcttaatgg tgatgtacgc tacggcttcc ggcttgattt cttgcaggaa
      661 ctggaaggct gtcgggcgct tgccgcgctt agc
//
