LOCUS       sequence-99394-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning PAmCherry1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Nan X, Collisson EA, Lewis S, Huang J, Tamguney TM, Liphardt JT, 
            McCormick F, Gray JW, Chu S
  TITLE     Single-molecule superresolution imaging allows quantitative analysis
            of RAF multimer formation and signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18519-24. doi: 
            10.1073/pnas.1318188110. Epub 2013 Oct 24.
  PUBMED    24158481
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1318188110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-11-12- 12"; volume: "110"; issue: "46"; pages: 
            "18519-24"
COMMENT     Sequence Label: pENTR-D-TOPO-PAmCherry1-MCS
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     164..180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     164..180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     185..204
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        185..203
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    221..320
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(651..670)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     920..938
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 catgttggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca taacacccct
       61 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
      121 tgcaatgtaa catcagagat tttgagacac gggccagagc tgccaggaaa cagctatgac
      181 catgtaatac gactcactat aggggatatc agctggatgg caaataatga ttttattttg
      241 actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta taatgccaac
      301 tttgtacaag aaagctgggt cggcgcgccc acccttttag gatccgaatt cgtcgaccca
      361 tggcatatga gatctcttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
      421 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgcg
      481 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
      541 ctcagcgtcg tagtggccgc cgtccttcag cttgaccctt ggcttgacct cgcccttcag
      601 ggcgccgtcc tcggggtaca tccgctcgga gagggcctcc cagcccatgg tcttcttctg
      661 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
      721 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaacttcat
      781 cacgcgctcc cacttgaagc cctcggggaa ggacagcttg aagtagtcgg ggatgtcggc
      841 ggggtgcttc acgtaggcgt tggagccgta catgaactga ggggacagga tgtcccaggt
      901 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
      961 gcggccctcg ccctcgccct cg
//
