LOCUS       sequence-99397-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enhance solubility and correct disulfide bond-formation of fused 
            protein expressed in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, Nguyen MT, Vu TT, Jeong 
            B, Kim SW, Choe H
  TITLE     Expression and purification of biologically active human FGF2 
            containing the b'a' domains of human PDI in Escherichia coli.
  JOURNAL   Appl Biochem Biotechnol. 2013 May;170(1):67-80. doi: 
            10.1007/s12010-013-0140-3. Epub 2013 Mar 8.
  PUBMED    23471584
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12010-013-0140-3"; journalName: "Appl Biochem Biotechnol";
            date: "2013-05"; volume: "170"; issue: "1"; pages: "67-80"
COMMENT     Sequence Label: pDEST-hPDIb′a′
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..235)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             249..266
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(319..337)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      333..380
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(504..523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     714..735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      121 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      181 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatgt
      241 acctcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      301 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      361 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      421 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      481 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      541 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      601 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      661 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      721 cttgttccaa actggaacaa cactca
//
