LOCUS       sequence-99398-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Nickerson A, Huang T, Lin LJ, Nan X
  TITLE     Photoactivated localization microscopy with bimolecular fluorescence
            complementation (BiFC-PALM) for nanoscale imaging of protein-protein
            interactions in cells.
  JOURNAL   PLoS One. 2014 Jun 25;9(6):e100589. doi: 
            10.1371/journal.pone.0100589. eCollection 2014.
  PUBMED    24963703
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-25"; volume: "9(6):e100589. doi"; pages: " 
            10.1371/journal.pone.0100589. eCollection 2014"
COMMENT     Sequence Label: pENTR-D-TOPO-PAmCherry1_1-159-MCS
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     166..182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     166..182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     187..206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(386..405)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     655..673
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tccatgttgg aatttaatcg cggcctcgac gtttcccgtt gaatatggct cataacaccc
       61 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
      121 tgtgcaatgt aacatcagag attttgagac acgggccaga gctgccagga aacagctatg
      181 accatgtaat acgactcact ataggggata tcagctggat ggcaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct tataatgcca
      301 actttgtaca agaaagctgg gtcggcgcgc ccaccctttt agtcctcggg gtacatccgc
      361 tcggagaggg cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
      421 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
      481 gtcacggtca ccacgccgcc gtcctcgaac ttcatcacgc gctcccactt gaagccctcg
      541 gggaaggaca gcttgaagta gtcggggatg tcggcggggt gcttcacgta ggcgttggag
      601 ccgtacatga actgagggga caggatgtcc caggtgaagg gcagggggcc acccttggtc
      661 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
      721 tcgaacacgt ggccgttcac ggagccctcc atatgcacct tgaagcgcat gaactccttg
      781 atgatggcca tgttatcctc ctcgcccttg ctcaccatgg taccgagctc ctgcagctcg
      841 agaagctt
//
