LOCUS       sequence-99399-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Nickerson A, Huang T, Lin LJ, Nan X
  TITLE     Photoactivated localization microscopy with bimolecular fluorescence
            complementation (BiFC-PALM) for nanoscale imaging of protein-protein
            interactions in cells.
  JOURNAL   PLoS One. 2014 Jun 25;9(6):e100589. doi: 
            10.1371/journal.pone.0100589. eCollection 2014.
  PUBMED    24963703
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-25"; volume: "9(6):e100589. doi"; pages: " 
            10.1371/journal.pone.0100589. eCollection 2014"
COMMENT     Sequence Label: pENTR-D-TOPO-PAmCherry1_160-236-MCS
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     166..182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     166..182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     187..206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tccatgttgg aatttaatcg cggcctcgac gtttcccgtt gaatatggct cataacaccc
       61 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
      121 tgtgcaatgt aacatcagag attttgagac acgggccaga gctgccagga aacagctatg
      181 accatgtaat acgactcact ataggggata tcagctggat ggcaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct tataatgcca
      301 actttgtaca agaaagctgg gtcggcgcgc ccaccctttt aggatccgaa ttcgtcgacc
      361 catggcatat gagatctctt gtacagctcg tccatgccgc cggtggagtg gcggccctcg
      421 gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg
      481 cggttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg
      541 acctcagcgt cgtagtggcc gccgtccttc agcttgaccc ttggcttgac ctcgcccttc
      601 agggcgccca tggtaccgag ctcctgcagc tc
//
