LOCUS       sequence-99406-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by PBAD. The C-terminal fragment has the point 
            mutation N748D. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-N748D
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(341..368)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atcatcgatg
       61 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
      121 agccgtcaat tgtctgattc gttaccaaca gacattgccg tcactgcgtc ttttactggc
      181 tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga
      241 ccaaagccat gacaaaaacg cgtaatttca acacgagcca aaataataaa aaagcaggat
      301 taataatctg gctttttata ttctctctct agtatataaa cgcagaaagg cccacccgaa
      361 ggtgagccag tgtgatccaa gccagctcga tatccgtcgg cttgaacgaa ttgttagaca
      421 ttacttgtag acgtgcccta cgcgcttgtt gagttgttcc tcaacgtttt tcttgaagtg
      481 cttagcttca aggtcacgga tacgaccgaa gcgagcctcg tcctcaatgg cccgaccgat
      541 tgcgcttgct acagcctgaa cggttgtatt gtcagcactg gttaggcaag ccagagtggt
      601 cttaatggtg atgtacgcta cggcttccgg cttgatttct tgcaggaact ggaaggctgt
      661 cgggcgcttg ccgcgcttag ctttcacttc ctcaaaccag tcgttgatgc gtgcaatcat
      721 c
//
