LOCUS       sequence-99415-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            R756S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-R756S
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttatcgca actctctact gtttctccat acccgttttt ttgggctagc actagtggag
       61 ccctatatat aaagatcagg gccaggtatg aaagcattta tgcaagttgt cgaggctgac
      121 atgctctcta agggtctact cggtggcgag gcgtggtctt cgtggcataa ggaagactct
      181 attcatgtag gagtacgctg catcgagatg ctcattgagt caaccggaat ggttagctta
      241 caccgccaaa atgctggcgt agtaggtcaa gactctgaga ctatcgaact cgcacctgaa
      301 tacgctgagg ctatcgcaac ccgtgcaggt gcgctggctg gcatctctcc gatgttccaa
      361 ccttgcgtag ttcctcctaa gccgtggact ggcattactg gtggtggcta ttgggctaac
      421 ggtcgtcgtc ctctggcgct ggtgcgtact cacagtaaga aagcactgat gcgctacgaa
      481 gacgtttaca tgcctgaggt gtacaaagcg attaacattg cgcaaaacac cgcatggaaa
      541 atcaacaaga aagtcctagc ggtcgccaac gtaatcacca agtggaagca ttgtccggtc
      601 gaggacatcc ctgcgattga gcgtgaagaa ctcccgatga aaccggaaga catcgacatg
      661 aatcctgagg ctctcaccgc gtggaaacgt gctgccgctg ctgtgtaccg caaggacaag
      721 gctcgcaagt ctcgccgtat cagccttgag ttcatgcttg agcaagccaa taagtttgct
      781 aaccataagg ccatctggtt cccttacaac atggactggc gcggtcgtg
//
