LOCUS       sequence-99416-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            R756S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-R756S
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca
       61 tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca
      121 ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg
      181 gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag
      241 caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga
      301 ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg
      361 tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt
      421 tcagcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg
      481 agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg
      541 tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg
      601 gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca
      661 catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg
      721 agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca
      781 tcttagagtc ggacttcgcg ttcgcgtaat ctagagtcga cctgcaggca tgcaagcttg
      841 gctgtt
//
