LOCUS       sequence-99418-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            N748D..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-N748D
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(162..189)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
       61 accaaagcca tgacaaaaac gcgtaatttc aacacgagcc aaaataataa aaaagcagga
      121 ttaataatct ggctttttat attctctctc tagtatataa acgcagaaag gcccacccga
      181 aggtgagcca gtgtgatcca agccagctcg atatccgtcg gcttgaacga attgttagac
      241 attacttgta gacgtgccct acgcgcttgt tgagttgttc ctcaacgttt ttcttgaagt
      301 gcttagcttc aaggtcacgg atacgaccga agcgagcctc gtcctcaatg gcccgaccga
      361 ttgcgcttgc tacagcctga acggttgtat tgtcagcact ggttaggcaa gccagagtgg
      421 tcttaatggt gatgtacgct acggcttccg gcttgatttc ttgcaggaac tggaaggctg
      481 tcgggcgctt gccgcgctta gctttcactt cctcaaacca gtcgttgatg cgtgcaatca
      541 tcttagggag tagggtagtg atgagaggct tggcggcagc gttatccgca acctcaccag
      601 ctttaagttg acgctcaaac atcttgcgga agcgtgcttc acccatctcg taagactcat
      661 gctcaagggc caactgttcg cgagctaaac gctcaccgta atggtcagcc agagtgttga
      721 acgggatagc agccagttcg atgtcagaga agtcgttctt agcgatgtta atcgtgttca
      781 tattagaaaa agaatgttat taag
//
