LOCUS       sequence-99419-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            N748D..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-N748D
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcactag tggagcccta
       61 tatataaaga tcagggccag gtatgaaagc atttatgcaa gttgtcgagg ctgacatgct
      121 ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca
      181 tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg
      241 ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc
      301 tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg
      361 cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg
      421 tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt
      481 ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa
      541 caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga
      601 catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc
      661 tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg
      721 caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca
      781 taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtc
//
