LOCUS       sequence-99420-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            N748D..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-N748D
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac gggtccaaag
       61 agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt gattccggca
      121 agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg
      181 aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt aagtctgctg
      241 ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt
      301 gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta
      361 ttcagacgcg cttggatctg atgttcctcg gtcagttccg cttacagcct accattaaca
      421 ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg
      481 tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg
      541 gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct gacgctgcga
      601 acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg
      661 ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag
      721 cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg
      781 cgtaatctag agtcgacctg caggcatgca agcttggctg
//
