LOCUS       sequence-99423-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:GACT:GFP
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..49)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     644..672
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(734..753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(735..753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca tgtggtctct
       61 cttttcgttg ggatctttcg aaagggcaga ttgtgtggac aggtaatggt tgtctggtaa
      121 aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgct
      181 tccatcttca atgttgtgtc taattttgaa gttaactttg attccattct tttgtttgtc
      241 tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc caagaatgtt
      301 tccatcttct ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc
      361 aaacttgact tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc
      421 ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgctgtttca tatgatctgg
      481 gtatctcgca aagcattgaa caccataacc gaaagtagtg acaagtgttg gccatggaac
      541 aggtagtttt ccagtagtgc aaataaattt aagggtaagt tttccgtatg ttgcatcacc
      601 ttcaccctct ccactgacag aaaatttgtg cccattaaca tcaccatcta attcaacaag
      661 aattgggaca actccagtga aaagttcttc tcctttacgc atgacgtcgg tatatctcct
      721 tcttaaagtt aaaccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      781 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgc
//
