LOCUS       sequence-99424-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:GACT:GFP
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     341..369
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(431..450)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(432..450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(534..553)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcttcttta aaatcaatac cttttaactc gattctatta acaagggtat caccttcaaa
       61 cttgacttca gcacgtgtct tgtagttccc gtcatctttg aaaaatatag ttctttcctg
      121 tacataacct tcgggcatgg cactcttgaa aaagtcatgc tgtttcatat gatctgggta
      181 tctcgcaaag cattgaacac cataaccgaa agtagtgaca agtgttggcc atggaacagg
      241 tagttttcca gtagtgcaaa taaatttaag ggtaagtttt ccgtatgttg catcaccttc
      301 accctctcca ctgacagaaa atttgtgccc attaacatca ccatctaatt caacaagaat
      361 tgggacaact ccagtgaaaa gttcttctcc tttacgcatg acgtcggtat atctccttct
      421 taaagttaaa ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta
      481 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
      541 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
      601 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
      661 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
      721 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa
      781 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attca
//
