LOCUS       sequence-99425-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:CACT:GFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(30..50)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     645..673
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(838..857)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
       61 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      121 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      181 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
      241 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt
      301 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
      361 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
      421 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
      481 ggtatctcgc aaagcattga acaccataac cgaaagtagt gacaagtgtt ggccatggaa
      541 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
      601 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
      661 gaattgggac aactccagtg aaaagttctt ctcctttacg catgacgtcg gtatatctcc
      721 ttcttaaagt taaaccctat agtgagtggt attaatttcg cgggatcgag atctcgatcc
      781 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      841 atatcgccga catcaccgat
//
