LOCUS       sequence-99428-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:CCCT:GFP
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     404..432
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(597..616)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc caagaatgtt
       61 tccatcttct ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc
      121 aaacttgact tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc
      181 ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgctgtttca tatgatctgg
      241 gtatctcgca aagcattgaa caccataacc gaaagtagtg acaagtgttg gccatggaac
      301 aggtagtttt ccagtagtgc aaataaattt aagggtaagt tttccgtatg ttgcatcacc
      361 ttcaccctct ccactgacag aaaatttgtg cccattaaca tcaccatcta attcaacaag
      421 aattgggaca actccagtga aaagttcttc tcctttacgc atgacgtcgg tatatctcct
      481 tcttaaagtt aaaccctata gtgaggggta ttaatttcgc gggatcgaga tctcgatcct
      541 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      601 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      661 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      721 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      781 cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca
      841 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattca
//
