LOCUS       sequence-99430-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:GCAT:GFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     328..356
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(521..540)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcaatacctt ttaactcgat tctattaaca agggtatcac cttcaaactt gacttcagca
       61 cgtgtcttgt agttcccgtc atctttgaaa aatatagttc tttcctgtac ataaccttcg
      121 ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat ctgggtatct cgcaaagcat
      181 tgaacaccat aaccgaaagt agtgacaagt gttggccatg gaacaggtag ttttccagta
      241 gtgcaaataa atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg
      301 acagaaaatt tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca
      361 gtgaaaagtt cttctccttt acgcatgacg tcggtatatc tccttcttaa agttaaaccc
      421 tatagtgatg cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
      481 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
      541 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
      601 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
      661 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
      721 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
      781 ggcatgatag cgcccggaag agagtcaatt ca
//
