LOCUS       sequence-99435-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:ACAT:GFP
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(33..53)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     648..676
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(841..860)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
       61 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
      121 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
      181 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
      241 tgtctgccat gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa
      301 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
      361 cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
      421 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat
      481 ctgggtatct cgcaaagcat tgaacaccat aaccgaaagt agtgacaagt gttggccatg
      541 gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat
      601 caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa
      661 caagaattgg gacaactcca gtgaaaagtt cttctccttt acgcatgacg tcggtatatc
      721 tccttcttaa agttaaaccc tatagtgatg tgtattaatt tcgcgggatc gagatctcga
      781 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      841 cctatatcgc cgacatcacc gat
//
