LOCUS       sequence-99436-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:ACAT:GFP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     446..474
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(639..658)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt
       61 gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc
      121 aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg
      181 tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg
      241 catggcactc ttgaaaaagt catgctgttt catatgatct gggtatctcg caaagcattg
      301 aacaccataa ccgaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt
      361 gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac
      421 agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga caactccagt
      481 gaaaagttct tctcctttac gcatgacgtc ggtatatctc cttcttaaag ttaaacccta
      541 tagtgatgtg tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt
      601 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
      661 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
      721 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
      781 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
      841 taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg
      901 catgatagcg cccggaagag agtcaattca
//
