LOCUS       sequence-99441-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for FAK fret sensor without helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCS2-FAK0
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..325
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     262..283
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatca gcgacaacgt
       61 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
      121 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      181 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
      241 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      301 tctcggcatg gacgagctgt acaagttaat taagggaagc ggaggaagcg gaggaagcgg
      361 aggaagcgga ccatcaacca gagattatga aattcaaagg gagagaattg aactggggcg
      421 ctgcattggt gaaggacagt ttggagatgt gcaccaagga atttacatga gtccggaaaa
      481 tccagctatg gctgtagcaa tcaaaacatg taaaaactgc acctcagaca gcgttagaga
      541 aaagttccta caagaagcct taacaatgcg tcagtttgat catcctcaca ttgtgaagct
      601 cattggagtt attacagaaa acccagtgtg gataatcatg gagctctgta cacttggaga
      661 gttgagatcg tttctgcaag taagaaaatt cagcttggac ctggcctccc tcatcctcta
      721 cgcttaccag cttagcacag cacttgctta cctagagagc aaaagatttg tacatagaga
      781 tattgctgct aggaacgtgc tggtatctgc cactgactgt gtgaaattgg gtgactttgg
      841 cttatcccga tacatggaag acagtactta ctataaggcg tccaaaggaa agttacctat
      901 caaatggatg gctccagagt caatcaactt ccgacggttt acctcagcaa gcgatgtgtg
      961 gatgtttggt gtgtgtatgt gggagatcct gatgcatggg gtaaag
//
