LOCUS       sequence-99443-e         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a.a. 417-887 of human ZO-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Nomme J, Fanning AS, Caffrey M, Lye MF, Anderson JM, Lavie A
  TITLE     The Src homology 3 domain is required for junctional adhesion 
            molecule binding to the third PDZ domain of the scaffolding protein 
            ZO-1.
  JOURNAL   J Biol Chem. 2011 Dec 16;286(50):43352-60. doi: 
            10.1074/jbc.M111.304089. Epub 2011 Oct 26.
  PUBMED    22030391
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.304089"; journalName: "J Biol Chem"; date: 
            "2011-12-16- 16"; volume: "286"; issue: "50"; pages: "43352-60"
COMMENT     Sequence Label: pET14b_tev_PSGU
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             141..158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             168..185
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggcgctagta ggatcgtagt atctcgatcc cccacgaccg caagcaagac gtcatcatgt
       61 agtcccgacg atggccgagc ccgatcttcc ccatcggtgt aggtttaact ttaagaagga
      121 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
      181 gcagccatat gattcttcgg cccagcatga aattggtaaa attcagaaaa ggagatagtg
      241 tgggtttgcg gctggctggt ggaaatgatg ttggaatatt tgtagctggc gttctagaag
      301 atagccctgc agccaaggaa ggcttagagg aaggtgatca aattctcagg gtaaacaacg
      361 tagattttac aaatatcata agagaagaag ccgtcctttt cctgcttgac ctccctaaag
      421 gagaagaagt gaccatattg gctcagaaga agaaggatgt ttatcgtcgc attgtagaat
      481 cagatgtagg agattctttc tatattagaa cccattttga atatgaaaag
//
