LOCUS       sequence-99444-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for FAK fret sensor without helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCS2-FAK0
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             677..697
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(758..779)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agtgctagaa aaagatgtcg gtttaagacg attttttccg aagagtttgc tagattcagt
       61 gaaggccaaa acactacgaa aattaatcca acagacattt cgacaatttg ccaacctcaa
      121 cagagaagaa agtattttga aattctttga gatcctctct ccagtgtaca gatttgacaa
      181 ggaatgcttc aagtgtgccc ttggttcaag ctggattatt tcagtggagc tggcaattgg
      241 cccagaggaa ggaatcagct accttacaga caagggtgca aatccaactc acctggcaga
      301 ttttaatcaa gtacaaacta ttcagtattc aaacagtgaa gacaaggaca gaaaagggat
      361 gttgcaactg aagatagctg gtgcacctga gcctctgaca gtgacagcac catccttaac
      421 cattgcagag aatatggctg acttgataga cggatactgc cgactggtga atggagccac
      481 gcaatctttt attatcaggc cacagaaaga aggtgaaaga gctttaccat caataccaaa
      541 gctggccaac aatgagaagc aaggagtaag gtcgcacaca gtctctgtat cagaaacaga
      601 tgactatgca gagataatag atgaagaaga tacttataca atgggaagcg gaggaagcgg
      661 agaattcgga agcggagaaa acctgtattt tcagggcgga agcggaggcg cgcccgtgag
      721 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      781 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      841 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      901 caccctgacc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      961 cttcttcaag tccgccatgc ccgaaggcta cgtcca
//
