LOCUS       sequence-99448-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FAK fret control sensor without FERM domain with 30 nm linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK-kinase30
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..51)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             191..442
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     379..400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             467..487
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
       61 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      121 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      181 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      241 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      301 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga gcaaagaccc
      361 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      421 cggcatggac gagctgtaca aggaattcgg aagcggagga agcggagaaa acctgtattt
      481 tcagggcgga agcggagaag aggaagagaa gaagaaagaa gaggaagaaa agaaacaaaa
      541 agaagaacaa gaaagacttg caaaagaaga ggcagagaga aaacaaaaag aagaacaaga
      601 aagacttgca aaagaagagg cagagagaaa acaaaaggag gaagaagaga gaaaacaaaa
      661 ggaagaagaa gagagaaaac aaaaggagga agaagaaaga aaattaaagg aggaacaaga
      721 aagaaaagct gcagaagaaa agaaagctaa agaagaagct gagagaaagg ctaaagaaga
      781 acaagaaagg aaagctgaag aagagagaaa gaagaaagaa gaggaagaaa gacttgaaag
      841 agaaagaaaa gagagagaag aacaagaaaa gaaagccaaa g
//
