LOCUS       sequence-9945-ex        8354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pIS1 DICER1 long-mut-miR103/107
FEATURES             Location/Qualifiers
     source          1..8354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     944..963
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1006..1024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1969
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             2120..2131
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6309..6430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6320..6339)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6374..6393
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6563..6667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6668..6736,6737..7528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6886..6905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7699..8287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8188..8207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
      361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
      421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
      481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
      541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
      601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
      661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
      721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
      781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
     1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
     1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
     1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
     1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
     1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
     1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
     1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
     1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
     1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
     1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
     1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
     1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
     1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
     1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
     1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
     1981 ctataccggt ctcgatatca ctactagtgt tctagaaacc gctttttaaa attcaaaaca
     2041 cgcaacaaaa caaaaaaaat taaggggaaa attatttaaa tcggaaagga agacttaaag
     2101 ttgttagtga gtggaatgaa ttgaaggcag aatttaaagt ttggttgata acaggataga
     2161 taacagactc aaacatttaa catatgtctc aaattttgga actaattgta gttttagttt
     2221 tttgcgcaaa cacaatctta tcttctttcc tcacttctgc tttgtttaaa tcacaagagt
     2281 gctttaatga tgacatttag caagtgctca aaataattga caggttttgt tttttttttt
     2341 ttgagtttat gtcagctttg cttagtgtta gaaggccatg gagcttaaac ctccagcagt
     2401 ccctaggatg atgtagattc ttctccatct ctccgtgtgt gcagtagtgc cagtcctgca
     2461 gtagttgata agctgaatag aaagataagg ttttcgagag gagaagtgcg ccaatgttgt
     2521 cttttctttc cacgttatac tgtgtaaggt gatgttcccg gtcgctgttg cacctgatag
     2581 taagggacag atttttaatg aacattggct ggcatgttgg tgaatcacat tttagttttc
     2641 tgatgccaca tagtcttgca taaaaaaggg ttcttgcctt aaaagtgaaa ccttcatgga
     2701 tagtctttaa tctctgatct ttttggaaca aactgtttta cattcctttc attttattat
     2761 gcattagacg ttgagacagc gtgatactta caactcacta gtatagttgt aacttattac
     2821 aggatcatac taaaatttct gtcatatgta tactgaagac attttaaaaa ccagaatatg
     2881 tagtctacgg atatttttta tcataaaaat gatctttggc taaacacccc attttactaa
     2941 agtcctcctg ccaggtagtt cccactgatg gaaatgttta tggcaaataa ttttgccttc
     3001 taggctgttg ctctaacaaa ataaacctta gacatatcac acctaaaata tgcgacagat
     3061 tttataattg attggttact tatttaagaa gcaaaacaca gcacctttac ccttagtctc
     3121 ctcacataaa tttcttacta tacttttcat aatgttgcat gcatatttca cctaccaaag
     3181 ctgtgctgtt aatgccgtga aagtttaacg tttgcgataa actgccgtaa ttttgataca
     3241 tctgtgattt aggtcattaa tttagataaa ctagctcatt atttccatct ttggaaaagg
     3301 aaaaaaaaaa aaacttcttt aggcatttgc ctaagtttct ttaattagac ttgtaggcac
     3361 tcttcactta aatacctcag ttcttctttt cttttgcatg catttttccc ctgtttggtg
     3421 ctatgtttat gtattatgct tgaaatttta attttttttt ttttgcactg taactataat
     3481 acctcttaat ttaccttttt aaaagctgtg ggtcagtctt gcactcccat caacatacca
     3541 gtagaggttt gcgacaattt gccccgttaa ttatgcttga agtttaagaa agctgagcag
     3601 aggtgtctca tatttcccag cacatgattc tgaacttgat gcttcgtgga atgcgacatt
     3661 tatatgtaag tgacatttga atactgtcct tcctgcttta tctgcatcat ccacccacag
     3721 agaaatgcct ctgtgcgagt gcaccgacag aaaactgtca gctctgcttt ctaaggaacc
     3781 ctgagtgagg ggggtattaa gcttctccag tgttttttgt tgtctccaat cttaaactta
     3841 aattgagatc taaattatta aacgagtttt tgagcaaatt aggtgacttg ttttaaaaat
     3901 atttaattcc gatttggaac cttagatgtc tatttgattt tttaaaaaac cttaatgtaa
     3961 gatatgacca gttaaaacaa agcaattctt gaattatata actgtaaaag tgtgcagtta
     4021 acaaggctgg atgtgaattt tattctgagg gtgatttgtg atcaagttta atcacaaatc
     4081 tcttaatatt tataaactac ctgatgccag gagcttaggg ctttgcattg tgtctaatac
     4141 attgatccca gtgttacggg attctcttga ttcctggcac caaaatcaga ttgttttcac
     4201 agttatgatt cccagtggga gaaaaatgcc tcaatatatt tgtaacctta agaagagtat
     4261 ttttttgtta atactaagat gttcaaactt agacatgatt aggtcataca ttctcagggg
     4321 ttcaaatttc cttctaccat tcaaatgttt tatcaacagc aaacttcagc cgtttcactt
     4381 tttgttggag aaaaatagta gattttaatt tgactcacag tttgaagcat tctgtgatcc
     4441 cctggttact gagttaaaaa ataaaaaagt acgagttaga catatgaaat ggttatgaac
     4501 gcttttgtgc tgctgatttt taatgctgta aagttttcct gtgtttagct tgttgaaatg
     4561 ttttgcatct gtcaattaag gaaaaaaaaa atcactctat gttgccccac tttagagccc
     4621 tgtgtgccac cctgtgttcc tgtgattgca atgtgagacc gaatgtaata tggaaaacct
     4681 accagtgggg tgtggttgtg ccctgagcac gtgtgtaaag gactggggag gcgtgtcttg
     4741 aaaaagcaac tgcagaaatt ccttatgatg attgtgtgca agttagttaa catgaacctt
     4801 catttgtaaa ttttttaaaa tttcttttat aatatgcttt ccgcagtcct aactatgcga
     4861 cgttttataa tagctttttc ccttctgttc tgttcatgta gcacagataa gcattgcact
     4921 tggtaccatg ctttacctca tttcaagaaa atatgcttaa cagagaggaa aaaaatgtgg
     4981 tttggccttg ctgctgtttt gatttatgga atttgaaaaa gataattata atgcctgcaa
     5041 tgtgtcatat actcgcacaa cttaaatagg tcatttttgt ctgtggcatt tttactgttt
     5101 gtgaaagtat gaaacagatt tgttaactga actcttaatt atgtttttaa aatgtttgtt
     5161 atatttcttt tcttttttct tttatattac gtgaagtgat gaaatttaga atgacctcta
     5221 acactcctgt aattgtcttt taaaatactg atatttttat ttgttaataa tactttgccc
     5281 tcagaaagat tctgataccc tgccttgaca acatgaaact tgaggctgct ttggttcatg
     5341 aatccaggtg ttcccccggc agtcggcttc ttcagtcgct ccctggaggc aggtgggcac
     5401 tgcagaggat cactggaatc cagatcgagc gcagttcatg cacaaggccc cgttgattta
     5461 aaatattgga tcttgctccg ttagggtgcc taatcccttt acacaagatt gaagccacca
     5521 aactgagacc ttgatacctt tttttaactg catctgaaat tatgttaaga gtctttaacc
     5581 catttgcatt atctgcagaa gagaaactca tgtcatgttt attacctata tggttgtttt
     5641 aattacattt gaataattat atttttccaa ccactgatta cttttcagga atttaattat
     5701 ttccagataa atttctttat tttatattgt acatgaaaag ttttaaagat atgtttaaga
     5761 ccaagactat taaaatgatt tttaaagttg ttggagacgc caatagcaat atctaggaaa
     5821 tttgcattga gaccattgta ttttccacta gcagtgaaaa tgatttttca caactaactt
     5881 gtaaatatat tttaatcatt acttcttttt ttctagtcca tttttatttg gacatcaacc
     5941 acagacaatt taaattttat agatgcacta agaattcact gcagcagcag gttacatagc
     6001 aaaaatgcaa aggtgaacag gaagtaaatt tctggctttt ctgctgtaaa tagtgaagga
     6061 aaattactaa aatcaagtaa aactaatgca tattatttga ttgacaataa aatatttacc
     6121 atcacatgcg acagctgttt tttaaggaac atgatgtcat tcattcatac agtaatcatg
     6181 cgacagaaat ttgcagtctg caccttatgg atcacaatta cctttagttg ttttttttgt
     6241 aataattgta gccaagtaaa tctccaataa agttatcgtc tgttcgcggc cgcttcgagc
     6301 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6361 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6421 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     6481 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccagg
     6541 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6601 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6661 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6721 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6781 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6841 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6901 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6961 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7021 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7081 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7141 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7201 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7261 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7321 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7381 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7441 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7501 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7561 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7621 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8341 tcacatggct cgac
//
