LOCUS       sequence-99451-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FAK fret control sensor without FERM domain with 30 nm linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK-kinase30
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(341..343,344..856)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(383..404)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(644..663)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agagagaaag aagaaagaag aggaagaaag acttgaaaga gaaagaaaag agagagaaga
       61 acaagaaaag aaagccaaag aagaggcaga gagaattgca aagttagagg ctgaaaagaa
      121 ggcagaagaa gaaagaaaag ccaaagaaga agaagagaga aaagccaaag aagaagagga
      181 aagaaagaag aaagaggagc aagaaagact tgcaaaagaa aaggaagaag cagaaagaaa
      241 agctgcagag gaaaagaaag ctaaagaaga acaagaaaga aaagaaaagg aagaagcaga
      301 aagaaaacaa agaggctctg gcggctctgg cggcgcgccc gtgagcaagg gcgaggagct
      361 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      421 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      481 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
      541 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      601 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      661 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      721 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcag
      781 cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      841 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      901 catcggcgac ggccccgtgc tgctgcccga caaccactac ct
//
