LOCUS       sequence-99461-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA and Cas9-Puro in Drosophila S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Bassett AR, Tibbit C, Ponting CP, Liu JL
  TITLE     Mutagenesis and homologous recombination in Drosophila cell lines 
            using CRISPR/Cas9.
  JOURNAL   Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 
            10.1242/bio.20137120.
  PUBMED    24326186
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120."
COMMENT     Sequence Label: pAc-sgRNA-Cas9
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             637..684
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             700..753
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
       61 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
      121 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
      181 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
      241 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
      301 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
      361 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
      421 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
      481 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
      541 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
      601 gagacacgga tcgacctgtc tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag
      661 gccggccagg caaaaaagaa aaagggatca ggcaagcttg agggcagagg aagtcttcta
      721 acatgcggtg acgtggagga gaatcccggc cctg
//
