LOCUS       sequence-99468-e         230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 system gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Zheng Q, Cai X, Tan MH, Schaffert S, Arnold CP, Gong X, Chen CZ, 
            Huang S
  TITLE     Precise gene deletion and replacement using the CRISPR/Cas9 system 
            in human cells.
  JOURNAL   Biotechniques. 2014 Sep 1;57(3):115-24. doi: 10.2144/000114196. 
            eCollection 2014.
  PUBMED    25209046
REFERENCE   2  (bases 1 to 230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114196";
            journalName: "Biotechniques"; date: "2014-09-1- 1"; volume: "57"; 
            issue: "3"; pages: "115-24"
COMMENT     Sequence Label: pUC-H1-gRNA
FEATURES             Location/Qualifiers
     source          1..230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..161
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 gaattcgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg
       61 cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg
      121 gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg
      181 tctttggatt tgggaatctt ataagttctg tatgaggacc acagatcccc
//
