LOCUS       sequence-99480-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy Esp3I-ccdB A-B
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     566..585
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(705..711,712..729,730..735))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
       61 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      121 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      181 atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat tctcaataaa
      241 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      301 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
      361 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      421 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      481 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      541 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      601 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      661 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      721 agcataaagt gtaaagcctg gggtgcctaa tgcggcccct cgaggggccg ccatagtgac
      781 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      841 atattgatat ttatatcatt ttacgtttct cgttcagctt tggtctcccc gcagagacga
      901 cga
//
