LOCUS       sequence-99481-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H83L mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)H83L
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(613..633)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(637..654)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             665..687
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    706..722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agctttcacg gggacatcac
       61 ctcctttggc tgggctttct tgcattccac cattttgttg ttgatttcat ggaagtggat
      121 ctcacaaact ttctctacga tgtcctcgct ctcaaacgtg acaaatccaa accctctgtg
      181 cctgttggtg gttttgtcga acatcagcat ggcatcatcc acctttccga actgctcgaa
      241 atagtgtttc acatcttcca ccgtggtgtt cacagacagc ccccccacga agatcttctt
      301 cgtccgagtg accatcttag gctgtgctct tcgaggaaag gccaccttgg ggtcaattgt
      361 tttggagtcg agctccagcc gagattgcgc cagcacttta tccacccccg cctggtccat
      421 gaaagtgacg aagccgaaac ccctggatct tttggtcagg gggtcccgca tcaccagaca
      481 ctctttcacc tccccgaact ggccgaagta ttcgcgcagc ccttcctgcg tggtctgcca
      541 actgagtcct ccgatgaaca tcttgcaggg gtcgtgcggc gagtccgggg aggcgaggcc
      601 gggctgggat ccgccctgaa aatacaggtt ttcgccgtga tgatgatgat gatgcatatg
      661 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      721 aa
//
