LOCUS       sequence-99483-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G64A mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)G64A
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(621..641)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(645..662)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             673..695
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    710..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(734..753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(735..753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag ctttcacggg
       61 gacatcacct cctttggctg agctttcttg cattccacca ttttgttgtt gatttcatgg
      121 aagtggatct cacacacttt ctctacgatg tcctcactct cgaacgtgac aaacccgaac
      181 cctctgtgcc tgttggtggt tttgtcgaac atcagcatgg catcatccac ctttccgaac
      241 tgctcaaaat agtgcttcac gtcctccacc gtggtgttca cagacagccc ccccacaaag
      301 atcttcttag ttcgagtgac catcttaggc tgtgctctcc gaggaaaggc caccttgggg
      361 tcaattgttt tggagtcgag ctcgtgccga gattgcgcca gcactttatc cacccccgcc
      421 tggtccatga aagtgacgaa ggcgaaaccc ctggatcttt tggtcagggg gtcccgcatc
      481 accagacact ctttcacctc cccgaactgg ccgaagtatt cgcgcagccc ttcctgcgtg
      541 gtctgccaac tgagtcctcc gatgaacatc ttgcaggggt cgtgcggcga gtccggggag
      601 gcgaggccgg gctgggatcc gccctgaaaa tacaggtttt cgccgtgatg atgatgatga
      661 tgcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      721 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      781 ctacgccgga cgcatcgtgg ccggcatcaa
//
