LOCUS       sequence-99486-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  R53E mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)R53E
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(618..638)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(642..659)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    708..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt tcacggggac
       61 atcacctcct ttggctgggc tttcttgcat tccaccattt tgttgttgat ttcatggaag
      121 tggatctcac aaactttctc tacgatgtcc tcgctctcaa acgtgacaaa tccaaaccct
      181 ctgtgcctgt tggtggtttt gtcgaacatc agcatggcat catccacctt tccgaactgc
      241 tcgaaatagt gtttcacatc ttccaccgtg gtgttcacag acagcccccc cacgaagatc
      301 ttcttcgtcc gagtgaccat cttaggctgt gctcttcgag gaaaggccac cttggggtca
      361 attgttttgg agtcgagctc gtgccgcgat tgcgccagca ctttatccac ccccgcctgg
      421 tccatgaaag tgacgaagcc gaaacccctg gatcttttgg tcagggggtc ttccatcacc
      481 agacactctt tcacctcccc gaactggccg aagtattcgc gcagcccttc ctgcgtggtc
      541 tgccaactga gtcctccgat gaacatcttg caggggtcgt gcggcgagtc cggggaggcg
      601 aggccgggct gggatccgcc ctgaaaatac aggttttcgc cgtgatgatg atgatgatgc
      661 atatgtatat ctccttctta aaagttaaac aaaattattt ctagagggga attgttatcc
      721 gctcacaatt cccctatagt
//
