LOCUS       sequence-99487-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  R53E R61E double mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)R53E/R61E
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(623..643)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(647..664)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agctttcacg
       61 gggacatcac ctcctttggc tgagctttct tgcattccac cattttgttg ttgatttcat
      121 ggaagtggat ctcacacact ttctctacga tgtcctcact ctcgaacgtg acaaacccga
      181 accctctgtg cctgttggtg gttttgtcga acatcagcat ggcatcatcc acctttccga
      241 actgctcaaa atagtgcttc acgtcctcca ccgtggtgtt cacagacagc ccccccacaa
      301 agatcttctt agttcgagtg accatcttag gctgtgctct ccgaggaaag gccaccttgg
      361 ggtcaattgt tttggagtcg agctcgtgcc gagattgcgc cagcacttta tccacccccg
      421 cctggtccat gaaagtgacg aagccgaaac cctcggatct tttggtcagg gggtcctcca
      481 tcaccagaca ctctttcacc tccccgaact ggccgaagta ttcgcgcagc ccttcctgcg
      541 tggtctgcca actgagtcct ccgatgaaca tcttgcaggg gtcgtgcggc gagtccgggg
      601 aggcgaggcc gggctgggat ccgccctgaa aatacaggtt ttcgccgtga tgatgatgat
      661 gatgcatatg tatatctcct tcttaaa
//
