LOCUS       sequence-9949-ex       12620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12620)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 12620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pMSCV Cyclin D2-long-mut
FEATURES             Location/Qualifiers
     source          1..12620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        7763..8262
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7798..7821)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8195..8214
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8283..8882
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8283..8302)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8779..8799
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8982..9496
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(9665..9681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9665..9681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9678..9700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9689..9705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9713..9719,9720..9737,9738..9743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9758..9779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9896..9913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10067..10655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10147..10166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10826..11617,11618..11686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11449..11468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11687..11791)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11859..11877
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11915..11937)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12037..12056
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12250..12272
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12400..12419
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(12619..12620,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatcttattt agccaaagga aggaggtcag
     1441 gggaacgctc tcccctcccc ttccaaaaaa caaaaacaga aaaacccttt tccaggccgg
     1501 ggaaagcagg agggagaggg gccgccgggc tggccatgga gctgctgtgc cacgaggtgg
     1561 acccggtccg cagggccgtg cgggaccgca acctgctccg agacgaccgc gtcctgcaga
     1621 acctgctcac catcgaggag cgctaccttc cgcagtgctc ctacttcaag tgcgtgcaga
     1681 aggacatcca accctacatg cgcagaatgg tggccacctg gatgctggag gtctgtgagg
     1741 aacagaagtg cgaagaagag gtcttccctc tggccatgaa ttacctggac cgtttcttgg
     1801 ctggggtccc gactccgaag tcccatctgc aactcctggg tgctgtctgc atgttcctgg
     1861 cctccaaact caaagagacc agcccgctga ccgcggagaa gctgtgcatt tacaccgaca
     1921 actccatcaa gcctcaggag ctgctggagt gggaactggt ggtgctgggg aagttgaagt
     1981 ggaacctggc agctgtcact cctcatgact tcattgagca catcttgcgc aagctgcccc
     2041 agcagcggga gaagctgtct ctgatccgca agcatgctca gaccttcatt gctctgtgtg
     2101 ccaccgactt taagtttgcc atgtacccac cgtcgatgat cgcaactgga agtgtgggag
     2161 cagccatctg tgggctccag caggatgagg aagtgagctc gctcacttgt gatgccctga
     2221 ctgagctgct ggctaagatc accaacacag acgtggattg tctcaaagct tgccaggagc
     2281 agattgaggc ggtgctcctc aatagcctgc agcagtaccg tcaggaccaa cgtgacggat
     2341 ccaagtcgga ggatgaactg gaccaagcca gcacccctac agacgtgcgg gatatcgacc
     2401 tgtgaggatg ccagttgggc cgaaagagag agacgcgtcc ataatctggt ctcttcttct
     2461 ttctggttgt ttttgttctt tgtgttttag ggtgaaactt aaaaaaaaaa ttctgccccc
     2521 acctagatca tatttaaaga tcttttagaa gtgagagaaa aaggtcctac gaaaacggac
     2581 tcataaaaag catttggtgc ctatttgaag tacagcataa gggaatccct tgtatatgcg
     2641 aacagttatt gtttgattat gtaaaagtaa tagtaaaatg cttacaggaa aacctgcaga
     2701 gtagttagag aatatgtatg cctgcaatat gggaacaaat tagaggagac tttttttttt
     2761 catgttatga gctagcacat acaccccctt gtagtataat ttcaaggaac tgtgtacgcc
     2821 atttatggca tgattagatt gcaaagcaat gaactcaaga aggaattgaa ataaggaggg
     2881 acatgatggg gaaggagtac aaaacaatct ctcaacatga ttgaaccatt tgggatggag
     2941 aagcaccttt gctctcagcc acctgttact aagtcaggag tgtagttgga tctctacatt
     3001 aatgtcctct tgctgtctac agtagcggat acctaaaaaa agatgtttta ttttgccagt
     3061 tggacacagg tgattggctc ctgggtttca tgttctgtga catcctgctt cttcttccaa
     3121 atgcagttca ttgcagacac caccatattg ctatctaatg gggaaatgta gctatgggcc
     3181 ataaccaaaa ctcacatgaa acggaggcag atggagacca agggtgggat ccagaatgga
     3241 gtcttttctg ttattgtatt taaaagggta atgtggcctt ggcatttctt cttagaaaaa
     3301 aactaatttt tggtgctgat tggcatgtct ggttcacagt ttagcattgt tataaaccat
     3361 tccattcgaa aagcactttg aaaaattgtt cccgagcgat agatgggatg gtttatgcaa
     3421 gtcatgctga atactcctcc cctcttctct tttgccccct cccttcctgc ccccagtctg
     3481 ggttactctt cgcttctggt atctggcgtt ctttggtaca cagttctggt gttcctacca
     3541 ggactcaaga gacacccctt cctgctgaca ttcccatcac aacattcctc agacaagcct
     3601 gtaaactaaa atctgttacc attctgatgg cacagaagga tcttaattcc catctctata
     3661 cttctccttt ggacatggaa agaaaagtta ttgctggtgc aaagatagat ggctgaacat
     3721 cagggtgtgg cattttgttc ccttttccgt tttttttttt tttattgttg ttgttaattt
     3781 tattgcaaag ttgtattcag cgtacttgaa tttttcttcc tctccacttc ttagaggcat
     3841 tcagttagca aagaggttgg agcaacaact tttttttttt tttttgcaca attgtaattg
     3901 acaggtaatg aagctatttg ttaaaatatt tgccttttta agtaaaaaag aaaaatcaga
     3961 acagggctat ttgaagaatt attttataca cagattctgc cttgtttcat agtatgaggg
     4021 ttgaagacgg aaaacaatct aagggtctct cattttttta attttgtttt gttcagtttg
     4081 gttttttttt ttttttgcgc ggataagaag ctaaagtcat ccatccttat tcacgttgac
     4141 agtacctagc tgtaatgttt cacagagtgt gcggatattt tataaacatt tttataatat
     4201 attattttac tgcttaaatt ccaagtcctg aagtagatgg ttgagatatg agttcttcgt
     4261 actggaaaag cccttccgta gtttgttttc ttctggtagc atattcatgg ttgttttttt
     4321 ttttcttttt tggttttttg gttttttttt tttcctctga tcacattctt caaagacgga
     4381 gtattcttta acgcaggttt actggacaaa atcaataact acaaaaggca atgattcacg
     4441 cttttgtttt cataataacg cacaaccgta cagtttctgc ttgggagccc attcgcatga
     4501 ggaatacaga agcagtgtga gcagggctga ctccctctca ggtggaaggc agggcggtct
     4561 cactcccagg gacctttttg gtcatggagg ccatcgggct cccagttaga ccctggtatc
     4621 ctcatcatga tggaaaaaat acattgaacc aagggatcct ccctcccctt caaggcagac
     4681 gttcagtaca aacatttatg cggtaggctc agatgtcgta atttgcactt aggtaccagg
     4741 tgtcaggaaa cagactaaaa agaattccac caggctgttt ggagatcctc atcttggagc
     4801 tttttcaaaa gcggggcttc atctgcaaag ggccctttca tcttgaagtt tttcccctcc
     4861 gtctttcccc tcccctggca tggacacctt gtgtttagga tcatctctgc aggtttccta
     4921 ggtctgaatc tgcgagtaga tgaacctgca gcaagcagcg tttatggtgc ttccttctcc
     4981 ctcctctgtc tcaaactgcg caggcaagca ctatgcaagc ccaggccctc tgctgagcgg
     5041 tactaaacgg tcgggttttc aatcacactg aattggcagg ataagaaaaa taggtcagat
     5101 aagtatggga tgatagttga agggaggtga agaggctgct tctctacaga ggtgaaattc
     5161 cagatgagtc agtctcttgg gaagtgtgtt tagaagggtt caggactttg tgagttagca
     5221 tgaccctaaa attctagggg atttctggtg ggacaatggg tggtgaattt tgaagttttg
     5281 gagagggaag tggagcagcc agcaagtaag ctagccagag ttttctcaag agccagcttt
     5341 gctcagcaca ctctcctggg ccccaaggag tcccacggaa tggggaaagt gggaaccctg
     5401 gagttcttgg gaatcttgga gcctaaagag aaaccgaggt gcaaattcat ttcatggtga
     5461 ctgacccttg agcttaaaca gaagcagcaa atgaaagaac cggacaaata aggaagggca
     5521 caagcctacc cgactctatt tacagtctgt aactttccac tcttcctgta gtcccgaggc
     5581 ccctgggtcc ttctagcttt tctctttccc atccttgggg ccttgtgtga tgatgggtgt
     5641 ggggctgccg atgggaaagt cgggggttgt taggcttttc tgcctgctcc tgcttaaaca
     5701 caagaaggaa tcctggattt tgccctctcc ttagctctta gtctctttgg taggagtttt
     5761 gttccagagg agctctcccc cttggatttg aacttgctct ttttgttgtt gttgttcttt
     5821 ctcttctttt tcttacctcc cactaaaggg gttccaaatt atcctggtct ttttctacct
     5881 tgttgtgttt ctatctcgtc tttacttcca tctgtttgtt tttttctcca tcagtggggg
     5941 ccgagttgtt cccccagcct gccaaatttt gatccttccc ctcttttggc caaatcctag
     6001 ggggaagaaa tcctagtatg ccaaaaatat atgctaagca taattaaact ccatgcgggt
     6061 ccataacagc caagaagcct gcaggagaaa gccaagggca gttccctccg cagaacaccc
     6121 catgcgtgct gagaggcgag ctccttgaag aaggggctgt tcttccagga ggccttattt
     6181 tgaactgcct caggacccca ctggagagca cagcatgcct tactactggg tcatccttgg
     6241 tctatgtgct ctgtactgga ggctctgttc tgcctcttat cagccaggtc aggggcacac
     6301 atggcttaag tgacaaagcc agaggagaag acaaccctga cagcatcacg ctgcatccca
     6361 ttgctagcag gattggcaac tcttcagacg gagctgcgct tccctgcagt ctagcacctc
     6421 tagggcctct ccagactgtg ccctgggagc tctgggactg aaaggttaag aacataaggc
     6481 aggatcagat gactctctcc aagagggcag gggaattttc tctccatggg ccacagggga
     6541 cagggctggg agaagaaata gacttgcacc ttatgtcatg taaataattg attttctagt
     6601 tcaagaagat aatattggta gtgtgggaat tggaggtagg aaggggagga agtctgagta
     6661 agccagttgg cttctaagcc aaaaggattc ctctttgttt atctctgaga cagtccaacc
     6721 ttgagaatag ctttaaaagg gaaattaatg ctgagatgat aaagtcccct taagccaaca
     6781 aaccctctgt agctatagaa tgagtgcagg tttctattgg tgtggactca gagcaattta
     6841 caagagctgt tcatgcagcc atccatttgt gcaaaatagg gtaagaagat tcaagaggat
     6901 atttattact tcctcatacc acatggcttt tgatgattct ggattctaaa caacccagaa
     6961 tggtcatttc aggcacaacg atactacatt cgtgtgtgtc tgcttttaaa cttggctggg
     7021 ctatcagacc ctattctcgg ctcaggtttt gagaagccat cagcaaatgt gtacgtgcat
     7081 gctgtagctg cagcctgcat cccttcgcct gcagcctact ttggggaaat aaagtgcctt
     7141 actgactgta gccattacag tatccaatgt cttttgacag gtgcctgtcc ttgaaaaaca
     7201 aagtttctat ttttattttt aattggttta gttcttaact gctggccaac tcttacatcc
     7261 ccagcaaatc atcgggccat tggatttttt ccattatgtt catcaccctt atatcatgta
     7321 acgcagatct ctctctctct cctctctctc agttatgtag tttcttgtct tggacttttt
     7381 ttttcttttc tttttctttt tttttttgct ttaaaacaag tgtgatgcca tatcaagtcc
     7441 atgttattct ctcacagtgt actctataag aggtgtgggt gtctgtttgg tcaggatgtt
     7501 agaaagtgct gataagtagc atgatcagtg tatgcgaaaa ggtttttagg aagtatggca
     7561 aaaatgttgt attggctatg atggtgacat gatatagtca gctgcctttt aagaggtctt
     7621 atctgttcag tgttaagtga tttaaaaaaa taataacctg ttttctgact agtttaaaga
     7681 tggatttgaa aatggttttg aatgcaatta ggttatgcta tttggacaat aaactcacct
     7741 tgacctgtta acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     7801 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     7861 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     7921 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     7981 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     8041 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     8101 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     8161 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     8221 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     8281 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8341 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     8401 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8461 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     8521 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8581 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8641 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8701 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8761 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8821 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8881 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga
     8941 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     9001 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag
     9061 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     9121 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     9181 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     9241 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     9301 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     9361 cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     9421 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     9481 agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag
     9541 gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa
     9601 tactcctgaa atagttcatt atggacagcg cagaagagct ggggagaatt aattcgtaat
     9661 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9721 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9781 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9841 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    10021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10501 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    11041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11821 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    11881 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    11941 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    12001 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    12061 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    12121 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    12181 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    12241 cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga
    12301 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
    12361 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
    12421 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggcg
    12481 attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt tgactcaaca
    12541 atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag
    12601 tctccagaaa aaggggggaa
//
