LOCUS       sequence-99495-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of activating sequence derived from native 
            galactose promoter coupled with a core promoter derived from native 
            Leucine promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASGAL-Pleum
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    394..511
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(572..592)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(572..590)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(611..627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(611..627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(624..646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    635..651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(659..665,666..683,684..689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    704..725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 catcaccatc taattcaacc aaaattggga caacaccagt gaataattct tcacctttag
       61 acatttttct agatagaatg gtatatcctt gaaatatata tatatatatt gctgaaatgt
      121 aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga aaaaacaata
      181 ggtccttaaa taatattggt ttaaacctgt taatagatca aaaatcatcg cttcgctgat
      241 taattacccc agaaataagg ctaaaaaact aatcgcatta tcatcctatg gttgttaatt
      301 tgattcgtta atttgaaggt ttgtggggcc aggttactgc caatttttcc tcttcataac
      361 cataaaagct agtattgtag aatctttatt gttcggagca gtgcggcgcg aggcacatct
      421 gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg aggagagtct tccgtcggag
      481 ggctgtcgcc cgctcggcgg cttctaatcc gtactaaagc ttccgttaat taaggggcgc
      541 gccaaggatc ccggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
      601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      721 cattaattgc gttgcgctca ctg
//
