LOCUS       sequence-99496-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of two gal binding sites coupled wiht a core 
            promoter derived from native Leucine promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS34-Pleum
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     191..208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    325..346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(361..366,367..384,385..391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    399..415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     404..426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     423..439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     423..439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     458..478
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        460..478
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
       61 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      121 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      181 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      241 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      301 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      361 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      421 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
      481 acaaaagctg gagctccggg atccttggcg cgccccttaa ttaacggaag cttcgggcga
      541 cagccctccg acggaagact ctcctccggt ttaaaccaat attatttaag gacctattgt
      601 tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt
      661 cagcaatata tatatatata tttcaaggat ataccattct atctagaaaa atgtctaaag
      721 gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt gatgttaatg
      781 gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tact
//
