LOCUS       sequence-99498-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis 
            with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal 
            tyrosine (delta Y).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis - human TUBA1a Cterm...
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             264..281
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agaactagtg gatccaaaat gagagaagtt attagtatta atggtatgtt cgatttgccc
       61 gtccaggcta gatctttttt ttgttagttc attttggcgt ttcatctttc attactaact
      121 tcaacgcaaa attttttttt ttttgatttc tctttacagt cggtcaagct ggttgtcaga
      181 ttggtaatgc ctgttgggaa ttatattccc ttgagcacgg tattaagccg gatggacatc
      241 tagaagatgg cctttcaaag ccgcatcatc accatcatca caagggagga gaagagggtt
      301 tctccacgtt tttccatgaa accggctacg gtaagttcgt tccaagggct atttacgtgg
      361 atttagagcc caacgttatt gacgaagtcc gtaacggtcc ttacaaggac ttgttccatc
      421 cagaacagtt gatcagtggt aaggaggacg cggctaataa ttatgcaaga ggccattaca
      481 ccgttggtag agaaattttg ggcgatgttc tggataggat tagaaaactg gcagaccaat
      541 gtgatgggtt acaagggttc ttgtttaccc attctcttgg tggtggtact ggttccggtc
      601 tagggtcctt actgttggaa gaactatccg ctgaatacgg taagaaatcc aagctggaat
      661 ttgccgtata ccctgctcca caagtgtcta cttctgtggt tgagccttac aacacagttt
      721 taactacaca tactacattg gaacatgcag attgtacttt catggtcgat aatgaggcta
      781 tttacgacat gtgc
//
