LOCUS       sequence-9950-ex        9780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pMSCV PIG IMP-1-short
FEATURES             Location/Qualifiers
     source          1..9780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        3562..4061
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3597..3620)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3994..4013
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4082..4681
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4082..4101)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4578..4598
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4741..5307
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4758..5309
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4902..4919)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5129..5148
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5321..5323,5324..5326,5327..6040)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5321..5323,5324..5326,5327..6037)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5366..5387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5627..5646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5974..5995
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6142..6656
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6825..6841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6825..6841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6838..6860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6849..6865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6873..6879,6880..6897,6898..6903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6918..6939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7056..7073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7227..7815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7307..7326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7986..8777,8778..8846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8609..8628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8847..8951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9019..9037
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9075..9097)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9197..9216
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9410..9432
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9560..9579
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9779..9780,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctatgaa caagctttac atcggcaacc
     1441 tcaacgagag cgtgaccccc gcggacttgg agaaagtgtt tgcggagcac aagatctcct
     1501 acagcggcca gttcttggtc aaatccggct acgccttcgt ggactgcccg gacgagcact
     1561 gggcgatgaa ggccatcgaa actttctccg ggaaagtaga attacaagga aaacgcttag
     1621 agattgaaca ttcggtgccc aaaaaacaaa ggagccggaa aattcaaatc cgaaatattc
     1681 caccccagct ccgatgggaa gtactggaca gcctgctggc tcagtatggt acagtagaga
     1741 actgtgagca agtgaacacc gagagtgaga cggcagtggt gaatgtcacc tattccaacc
     1801 gggagcagac caggcaagcc atcatgaagc tgaatggcca ccagttggag aaccatgccc
     1861 tgaaggtctc ctacatcccc gatgagcaga tagcacaggg acctgagaat gggcgccgag
     1921 ggggctttgg ctctcggggt cagccccgcc agggctcacc tgtggcagcg ggggccccag
     1981 ccaagcagca gcaagtggac atcccccttc ggctcctggt gcccacccag tatgtgggtg
     2041 ccattattgg caaggagggg gccaccatcc gcaacatcac aaaacagacc cagtccaaga
     2101 tagacgtgca taggaaggag aacgcaggtg cagctgaaaa agccatcagt gtgcactcca
     2161 cccctgaggg ctgctcctcc gcttgtaaga tgatcttgga gattatgcat aaagaggcta
     2221 aggacaccaa aacggctgac gaggttcccc tgaagatcct ggcccataat aactttgtag
     2281 ggcgtctcat tggcaaggaa ggacggaacc tgaagaaggt agagcaagat accgagacaa
     2341 aaatcaccat ctcctcgttg caagacctta ccctttacaa ccctgagagg accatcactg
     2401 tgaagggggc catcgagaat tgttgcaggg ccgagcagga aataatgaag aaagttcggg
     2461 aggcctatga gaatgatgtg gctgccatga gcctgcagtc tcacctgatc cctggcctga
     2521 acctggctgc tgtaggtctt ttcccagctt catccagcgc agtcccgccg cctcccagca
     2581 gcgttactgg ggctgctccc tatagctcct ttatgcaggc tcccgagcag gagatggtgc
     2641 aggtgtttat ccccgcccag gcagtgggcg ccatcatcgg caagaagggg cagcacatca
     2701 aacagctctc ccggtttgcc agcgcctcca tcaagattgc accacccgaa acacctgact
     2761 ccaaagttcg tatggttatc atcactggac cgccagaggc ccaattcaag gctcagggaa
     2821 gaatctatgg caaactcaag gaggagaact tctttggtcc caaggaggaa gtgaagctgg
     2881 agacccacat acgtgtgcca gcatcagcag ctggccgggt cattggcaaa ggtggaaaaa
     2941 cggtgaacga gttgcagaat ttgacggcag ctgaggtggt agtaccaaga gaccagaccc
     3001 ctgatgagaa cgaccaggtc atcgtgaaaa tcatcggaca tttctatgcc agtcagatgg
     3061 ctcaacggaa gatccgagac atcctggccc aggttaagca gcagcatcag aagggacaga
     3121 gtaaccaggc ccaggcacgg aggaagtgac cagcccctcc ctgtcccttc gagtccagga
     3181 caacaacggg cagaaatcga gagtgtgctc tccccggcag gcctgagaat gagtgggaat
     3241 ccgggacacc tgggccgggc tgtagatcag gtttgcccac ttgattgaga aagatgttcc
     3301 agtgaggaac cctgatctct cagccccaaa cacccaccca attggcccaa cactgtctgc
     3361 ccctcggggt gtcagaaatt ctagcgcaag gcacttttaa acgtggattg tttaaagaag
     3421 ctctccaggc cccaccaaga gggtggatca cacctcagtg ggaagaaaaa taaaatttcc
     3481 ttcaggtttt aaaaacatgc agagaggtgt tttaatcagc cttaaaggat ggttcatttc
     3541 tcgaggttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3601 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3661 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3721 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3781 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3841 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     3901 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     3961 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4021 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4081 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4141 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4201 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4261 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4321 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4381 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4441 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4501 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4561 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4621 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4681 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atcatccaat
     4741 tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4801 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4861 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4921 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4981 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     5041 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     5101 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     5161 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     5221 ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     5281 gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga
     5341 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5401 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5461 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5521 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5581 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5641 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5701 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5761 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5821 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5881 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5941 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     6001 cgccgggatc actctcggca tggacgagct gtacaagtaa tgaattaatt aagaattatc
     6061 aagcttatcg ataccgtcga cctgcagcca agcttatcga taaaataaaa gattttattt
     6121 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     6181 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     6241 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     6301 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     6361 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     6421 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     6481 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     6541 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     6601 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     6661 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     6721 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     6781 atggacagcg cagaagagct ggggagaatt aattcgtaat catggtcata gctgtttcct
     6841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6901 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6961 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7021 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7081 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7141 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7201 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7261 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7321 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7441 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7861 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7921 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7981 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8041 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8101 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8161 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8221 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8281 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8341 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8401 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8461 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8521 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8581 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8641 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8701 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8761 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8821 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8881 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8941 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9001 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9061 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9121 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9181 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     9241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     9301 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9361 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9421 cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     9481 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     9541 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     9601 cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag
     9661 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     9721 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
//
