LOCUS       sequence-99504-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of two gal binding sites coupled with a core 
            promoter derived from native Leucine promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS12-Pleum
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     190..207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    324..345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(360..365,366..383,384..390)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    398..414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     403..425
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     422..438
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     422..438
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     457..477
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        459..477
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
       61 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      181 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      241 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      301 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      361 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      421 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      481 caaaagctgg agctccggga tccttggcgc gccccttaat taacggaagc ttcggattag
      541 aagccgccga gggcgacagc cgtttaaacc aatattattt aaggacctat tgttttttcc
      601 aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat
      661 atatatatat atatttcaag gatataccat tctatctaga aaaatgtcta aaggtgaaga
      721 attattcact ggtgttgtcc
//
