LOCUS       sequence-99505-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of activating sequence derived from native 
            galactose promoter coupled with full length native galactose 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASGAL-Pgal
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..173
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    374..491
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 aaagctggag ctccgggatc cttggcgcgc cccttaatta acggaagctt tagtacggat
       61 tagaagccgc cgagcgggcg acagccctcc gacggaagac tctcctccgt gcgtcctcgt
      121 cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata
      181 aagattctac aatactagct tttatggtta tgaagaggaa aaattggcag taacctggcc
      241 ccacaaacct tcaaattaac gaatcaaatt aacaaccata ggatgataat gcgattagtt
      301 ttttagcctt atttctgggg taattaatca gcgaagcgat gatttttgat ctattaacag
      361 gtttaaacta gtacggatta gaagccgccg agcgggcgac agccctccga cggaagactc
      421 tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc
      481 gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa
      541 attggcagta acctggcccc acaaaccttc aaattaacga atcaaattaa caaccatagg
      601 atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga
      661 tttttgatct attaacagat atataaatgg aaaagctgca taaccacttt aactaatact
      721 ttcaacattt tcagtttgta ttac
//
