LOCUS       sequence-99514-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of activating sequence derived from native gal 
            promoter coupled with core promoter derived from native Leucine 
            promoter. Spacer between core and activating sequence was used. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASgal-A9-Pleum
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..134
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cccttaatta atagtacgga ttagaagccg ccgagcgggc gacagccctc cgacggaaga
       61 ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc
      121 gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga
      181 aaaattggca gtaacctggc cccacaaacc ttcaaattaa cgaatcaaat taacaaccat
      241 aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga
      301 tgatttttga tctattaaca gaagcttcat gtacttaccc aattaggaaa tttacatggt
      361 ttaaaccaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt
      421 actttctaac ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat
      481 ataccattct atctagaaaa atgtctaaag gtgaagaatt attcactggt gttgtcccaa
      541 ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg
      601 aaggtgatgc tacttacggt aaattgacct taaaatttat ttgtactact ggtaaattgc
      661 cagttccatg gccaacctta gtcactactt taggttatgg tttgatgtgt tttgctagat
      721 acccagatca ta
//
