LOCUS       sequence-9952-ex       16091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 16091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pMSCV PIG IMP-1-long-mut
FEATURES             Location/Qualifiers
     source          1..16091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        9873..10372
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9908..9931)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10305..10324
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10393..10992
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10393..10412)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10889..10909
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11052..11618
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    11069..11620
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11213..11230)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11440..11459
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(11632..11634,11635..11637,11638..12351)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11632..11634,11635..11637,11638..12348)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11677..11698)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11938..11957)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12285..12306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             12453..12967
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(13136..13152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13136..13152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13149..13171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13160..13176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13184..13190,13191..13208,13209..13214))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13229..13250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13367..13384)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13538..14126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13618..13637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14297..15088,15089..15157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14920..14939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15158..15262)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15330..15348
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15386..15408)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     15508..15527
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15721..15743
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15871..15890
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(16090..16091,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctatgaa caagctttac atcggcaacc
     1441 tcaacgagag cgtgaccccc gcggacttgg agaaagtgtt tgcggagcac aagatctcct
     1501 acagcggcca gttcttggtc aaatccggct acgccttcgt ggactgcccg gacgagcact
     1561 gggcgatgaa ggccatcgaa actttctccg ggaaagtaga attacaagga aaacgcttag
     1621 agattgaaca ttcggtgccc aaaaaacaaa ggagccggaa aattcaaatc cgaaatattc
     1681 caccccagct ccgatgggaa gtactggaca gcctgctggc tcagtatggt acagtagaga
     1741 actgtgagca agtgaacacc gagagtgaga cggcagtggt gaatgtcacc tattccaacc
     1801 gggagcagac caggcaagcc atcatgaagc tgaatggcca ccagttggag aaccatgccc
     1861 tgaaggtctc ctacatcccc gatgagcaga tagcacaggg acctgagaat gggcgccgag
     1921 ggggctttgg ctctcggggt cagccccgcc agggctcacc tgtggcagcg ggggccccag
     1981 ccaagcagca gcaagtggac atcccccttc ggctcctggt gcccacccag tatgtgggtg
     2041 ccattattgg caaggagggg gccaccatcc gcaacatcac aaaacagacc cagtccaaga
     2101 tagacgtgca taggaaggag aacgcaggtg cagctgaaaa agccatcagt gtgcactcca
     2161 cccctgaggg ctgctcctcc gcttgtaaga tgatcttgga gattatgcat aaagaggcta
     2221 aggacaccaa aacggctgac gaggttcccc tgaagatcct ggcccataat aactttgtag
     2281 ggcgtctcat tggcaaggaa ggacggaacc tgaagaaggt agagcaagat accgagacaa
     2341 aaatcaccat ctcctcgttg caagacctta ccctttacaa ccctgagagg accatcactg
     2401 tgaagggggc catcgagaat tgttgcaggg ccgagcagga aataatgaag aaagttcggg
     2461 aggcctatga gaatgatgtg gctgccatga gcctgcagtc tcacctgatc cctggcctga
     2521 acctggctgc tgtaggtctt ttcccagctt catccagcgc agtcccgccg cctcccagca
     2581 gcgttactgg ggctgctccc tatagctcct ttatgcaggc tcccgagcag gagatggtgc
     2641 aggtgtttat ccccgcccag gcagtgggcg ccatcatcgg caagaagggg cagcacatca
     2701 aacagctctc ccggtttgcc agcgcctcca tcaagattgc accacccgaa acacctgact
     2761 ccaaagttcg tatggttatc atcactggac cgccagaggc ccaattcaag gctcagggaa
     2821 gaatctatgg caaactcaag gaggagaact tctttggtcc caaggaggaa gtgaagctgg
     2881 agacccacat acgtgtgcca gcatcagcag ctggccgggt cattggcaaa ggtggaaaaa
     2941 cggtgaacga gttgcagaat ttgacggcag ctgaggtggt agtaccaaga gaccagaccc
     3001 ctgatgagaa cgaccaggtc atcgtgaaaa tcatcggaca tttctatgcc agtcagatgg
     3061 ctcaacggaa gatccgagac atcctggccc aggttaagca gcagcatcag aagggacaga
     3121 gtaaccaggc ccaggcacgg aggaagtgac cagcccctcc ctgtcccttc gagtccagga
     3181 caacaacggg cagaaatcga gagtgtgctc tccccggcag gcctgagaat gagtgggaat
     3241 ccgggacacc tgggccgggc tgtagatcag gtttgcccac ttgattgaga aagatgttcc
     3301 agtgaggaac cctgatctct cagccccaaa cacccaccca attggcccaa cactgtctgc
     3361 ccctcggggt gtcagaaatt ctagcgcaag gcacttttaa acgtggattg tttaaagaag
     3421 ctctccaggc cccaccaaga gggtggatca cacctcagtg ggaagaaaaa taaaatttcc
     3481 ttcaggtttt aaaaacatgc agagaggtgt tttaatcagc cttaaaggat ggttcatttc
     3541 ttgaccttaa tgtttttcca atcttcttcc ccctacttgg gtaattgatt aaaataacgc
     3601 catttacggc ctctttctat atttacacta atttttttat ctttattgct accagaaaaa
     3661 aatgcgaacg aatgcattgc tttgcttaca gtattgactc aagggaaaag aactgtcagt
     3721 atctgtagat taattccaat cactccctaa ccaataggta caatacggaa tgaagaagag
     3781 gggaaaatgg ggagaaagat ggttaaaata cataataatc cacgtttaaa aggagcgcac
     3841 ttgtggctga tctatgccag atcaccatct tcaaattggc acaactgaaa tttccccact
     3901 ctgttggggc ttccccacca cattcatgtc cctctcccgt gtaggtttca cattatgtcc
     3961 aggtgcacat aggtggtatt gaatgctcag cagggtaggg gctgaccact gtccctgatt
     4021 cccatcgttc tcaggcggat tttatatttt tttaaagtct attttaatga ttggatatga
     4081 gcactgggaa ggggacgcta actccccttg ataaagtctc ggttccatgg aggacttgag
     4141 tggccccaaa ggctgccacg gtgccctcac cccagcccat gtgctcccat aagggctggt
     4201 tcctagaggc aggggttgtg gggcactccc agccacggca ctgttacctt ggtggtggga
     4261 cttggaaccc aaccctgagc tcccgataaa gctaaagtcc atcatctggc aaattcagta
     4321 aattggagag tacttgcttc tgtttgtatc tgagaggaat ttttaactga cggcttctgt
     4381 ctccatgaat cattatcagc atgatgaaag gtgtgtctaa aaaacaattc agaataccag
     4441 cagcattgta cagcaagggg taaataagct taatttatta atttaccagg cttaattaag
     4501 atcccatgga gtgtttagcc cttgtgggag acagaagcca tcagttaaat gaggttaggc
     4561 ctctcctcct aatatactga ttgacaatgc atattagcca ggtaatgcac tttagctacc
     4621 ctggacaatg ctatcaagtg tgctgggaag ggaggaaggc ctctctacat atggaaaagc
     4681 ccatgcgtgg agttcccctc ctttcaacat tgcaacaaca gtaacaacaa gacaaccgca
     4741 acatgtgggc gtagtcaggc aatgctgtgt gcgaagtaaa ctaacgcaag gtatgaagtt
     4801 aacgcagcaa ttattttcct ttttgttccc cccaacccca ttaaaaaaat ttttttttga
     4861 tttttgtttt tttgcagctt gctgatattt tatataaaaa agaaaagcaa agcaaaagag
     4921 aagctgatag tcttgaatat tttatttttt taatgaaaag aaaaaacaag aaagttatgt
     4981 ttcataattt cttacaacat gagccagtaa ccctttagga actctctatg gagaacaggc
     5041 ctggtgggaa aggctttggg ggctgccccc ttaggaggag gctagtgcta agagggaagg
     5101 cccaggtttg agagagccca gaggggcaga gcccagagcc ttgtttggcc ctgatctctg
     5161 acttctagag ccccagctgc tggcggctgc tggaatatcc tacctgatag gattaaaagg
     5221 cctagtggag ctgggggctc tcagtggtta aacaatgccc aacaaccaac cagctggccc
     5281 ttggtctcct ctctttcctc ctttggttaa agagcatctc agccagcttt tcccaccagt
     5341 ggtgctgttg agatatttta aaatattgcc tccgttttat cgaggagaga aataataact
     5401 aaaaaatata ccctttaaaa aaacctatat ttctctgtct aaaaatatgg gagctgagat
     5461 tccgttcgtg gaaaaaagac aaggccaccc tctcgccctc agagaggtcc acctggtttg
     5521 tcattgcaat gcttttcatt tttttttttt gttattgttt catttcagtt ccgtcttgct
     5581 attcttccta atctatatcc atagatctaa ggggcaaaca gatactagtt aactgccccc
     5641 acctctgtct ccctgtcttc tttagatcgg tctgattgat tttaaaagtg gacccaaact
     5701 tagggaattc ttgatttagg gtggctggtg gcaaggaggg gcaggggata tggggacgtg
     5761 actgggacag gttcctgcct tatcattttc tccctaggac attcccttgt agcccccaga
     5821 attgtctggc ccaaattgaa tagaagcaga aaaacattta gggataacat caggccagta
     5881 gaattaagcc tctccacctg tcccaaccat aaaaagggtc tcccagcttt ccatctctgg
     5941 ctctatatgc tttatcccaa aacaaagcag ataacgttca gacgtcggcc atttagtaat
     6001 ttaaagcgaa tttccagcag caagcatgct ttgatatctg gttcagacta tcatcaggaa
     6061 gaaaaaaaaa tcccacagta cctgaaatgt gattgttgca gtgttcagtt tccttggggg
     6121 cctgctccct tcacaccttg agcccaagtc cttttccgtt ggctgattca gctcccagaa
     6181 gagacgagga agtgtgtggc aagggactgg aaaacttcac ttgcttggat taggcaaggc
     6241 tccactcatt gttgatattt gcccagcagg aaaatcatgt aagttatacc accagaaagc
     6301 aaaaggagca tggtttggtg gttaaggttt agtgggatga aggacctgtc ttggtgggcc
     6361 gggccctctt gtgccccgta ggctaggtct tagggcaact ccttgccctc ctgctcagca
     6421 cctccatttc cccatccttg gtgagataac aagctatcgc gaaaagcact tgggagattt
     6481 ggatgatttg agaagagtga cttaaaaaaa atgcttctgt gctctaagat atatatgtgt
     6541 gtgtgtgtgc tacatatata tttttaagaa aggaccatct ctttaggata tatttttaaa
     6601 ttctttgaaa cacataacca aaatggtttg attcactgac tgactttgaa gctgcatctg
     6661 ccagttacac cccaaatggc tttaatcccc tctcgggtct ggttgccttt tgcagtttgg
     6721 gttgtggact cagctcctgt gaggggtctg gttaggagag agccattttt aaggacaggg
     6781 agttttatag cccttttcta ctttcctccc ctcctcccag tccttatcaa tcttttttcc
     6841 tttttcctga ccccctcctt ctggaggcag ttgggagcta tccttgttta tgcctcacta
     6901 ttggcagaaa agaccccatt taaaacccag agaacactgg agggggatgc tctagttggt
     6961 tctgtgtcca ttttcctctg tgccaaagac agacagacag aggctgagag aggctgttcc
     7021 tgaatcaaag caatagccag ctttcgacac atacctggct gtctgaggag gaaggcctcc
     7081 tggaaactgg gagctaaggg cgaggccctt cccttcagag gctcctgggg gattagggtg
     7141 tggtgtttgc caagccaagg ggtagggagc cgagaaattg gtctgtcggc tcctggttgc
     7201 actttgggga aggagaggaa gtttggggct ccaggtagct ccctgttgtg ggactgctct
     7261 gtcccctgcc cctactgcag agatagcact gccgagttcc cttcaggcct ggcagacggg
     7321 cagtgaggag gggcctcagt tagctctcaa gggtgccttc ccctcctccc aacccagaca
     7381 taccctctgc caaactggga accagcagtg ctagtaacta acgcacagag ccccagaggg
     7441 cctgcttgag ccttcttgct ccacaggaga agctggtgcc tctaggcaac cccttcctcc
     7501 cacctctcat caggggtggg ggttctcctt tctttcccct gaagtgttta tggggagatc
     7561 ctagtggctt tgccattcaa accactcgac tgtttgcctg tttcttgaaa accagtagaa
     7621 gggaaacagc acagcctgtc acagtaattg caggaagatt gaagaaaaat cctcatcaat
     7681 gccaggggac ataaaagcca tttcccttcc aaatactcga caatttagat gcagaacatt
     7741 tctctgtatt cagacttaga gtaacaccag ctgaaaactg cagtttcttt cctttggata
     7801 cataaggctt ctctatcggg gtacgggaca gggaggaggc ctcatgtctg aagggggatt
     7861 taggggcgag agccccagcc ctgaccctcg gtcctgtgca ccgctttggg gcacagtctg
     7921 atggcgcctt tgctggcgcc ttagtatggt tgactccgga tggacaaaag aaaaaaaatt
     7981 ttttttcttg aatgaaatag caggaagctc ctcgggagca tgtgttttga ttaaccgcag
     8041 gtgatggatg ctacgagtat aaatggatta actaacgcaa tccttacagt aagattggaa
     8101 ctaagggcag ggactcatgc ataagggtat gaatcccagc caggacaagt gagttgaggc
     8161 ttgtgccaca aaaggtttgt ccttggggaa caggcaggcc tgccaggatc ccccccatat
     8221 cgattgggct gggagggctg gccatgaggt ccccactttc tgctttcctt gcccatgtgt
     8281 cacccctttg gcctccagct tgtccctctc tcactttcta tagctttgtt ggaccagatg
     8341 gtgaggaaag gaatggcctc ttcccttcta gagggggctg gctggagtga gacctggggc
     8401 ttggcctgga acccaccaca cagccccaaa gtcaggaagc ctggggaaac cagagctgag
     8461 acctcttcaa cagggtttct ttgagatcct acacctccat tgggcccttt ttcagtcttc
     8521 aatgggggcc cagttggctc tagaaggaga agaggtgaag caggatcctt tgccctgggg
     8581 gagtctgagg gcgcggtcct tggactcatt caggccgtct ttgtagttgg gggagttcca
     8641 ctgggcgatc ccagcccctc cccacccacc ctctaatgga cctcctcata gaagccccat
     8701 ttcacttttg ttttatctac ctcttagcaa aacaatagat aaattaggta gtggcagctc
     8761 cacttgctta ggttaggggg ggaaaaagat ttctttttcc aaaggaaaaa aatattacct
     8821 tgagaatact ttccaaaaaa taaaattaaa aaaaaaaaaa ccaaaaaaaa aaattttttt
     8881 ttaaaaggga gacattttcc agtgaccact ggattgtttt aatttcccaa gctttttttt
     8941 cccccataaa taagtttcac tctttggcga ttttcttcac ttgtttaaga taacgtgcta
     9001 gctattccaa caggtaacag ctttcacagt ctgcccctgg cctgtctcac cccatccccc
     9061 accctattcc tgccagtgag tccttcctgt gcttctctcc cttctcccct cccagccagc
     9121 tgacttcagt cacccctgtc ccccctcccc tgccaataag ctcccccagg aataaaggct
     9181 ttgttttggg gatgcttaaa tcttgactgg cacttcccgg ctgtgggggc tggggagcca
     9241 cttgtaacat ttctgtgcag attttatgtt agccactgct atgttaaagc acgttcaaaa
     9301 tgaatttcag cagattatgt gttaccataa tgaataaacg tcctctatca ccatttggag
     9361 tctccctttt ctccaggatc ttgatcctgg tccccaaaac cagagtgaat caaaagagct
     9421 tcctcccctg aggcaaagtg gatttgtaag cagttctgaa acatcactta ctcagaagag
     9481 ggaacgatgt attttgatga gtgcaaattg ggaagagctg gaggcctact gcttgggaca
     9541 gttttttttt tttttttttt tttaaatatg agtgctagct tattctgtaa ttgcggcaac
     9601 tttgaaaatt gtattttact ggaaatctgc cagccatcac cacccgattt tgattgtatc
     9661 cttcctccca tcctttaatc tgttcattgc tttgggggag gtggggcagc tggctcacac
     9721 gttggagttt gttctttgat ggatgaacga acactccagc tttctttccc gtgaaggttg
     9781 tttcagccac aaaccacttc attttgctgt ttcaatttca aaataaaagg aaacttatat
     9841 tgaaagacaa ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt tcccaaggca
     9901 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     9961 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
    10021 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
    10081 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
    10141 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
    10201 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
    10261 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
    10321 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
    10381 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
    10441 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
    10501 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
    10561 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
    10621 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
    10681 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
    10741 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
    10801 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
    10861 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
    10921 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
    10981 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
    11041 catcatccaa ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc
    11101 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
    11161 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
    11221 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
    11281 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
    11341 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
    11401 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
    11461 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
    11521 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
    11581 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
    11641 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
    11701 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
    11761 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
    11821 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
    11881 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
    11941 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
    12001 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
    12061 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
    12121 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
    12181 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
    12241 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
    12301 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta atgaattaat
    12361 taagaattat caagcttatc gataccgtcg acctgcagcc aagcttatcg ataaaataaa
    12421 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
    12481 agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
    12541 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
    12601 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
    12661 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
    12721 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
    12781 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata
    12841 gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
    12901 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
    12961 ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat
    13021 attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga
    13081 aatagttcat tatggacagc gcagaagagc tggggagaat taattcgtaa tcatggtcat
    13141 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    13201 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    13261 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    13321 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    13381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    13441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    13501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    13561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    13981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    14041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    14101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    14161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    14221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    14281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    14341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    14401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    14461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    14521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    14581 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    14641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    14701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    14761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    14821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    14881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    14941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    15001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    15061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    15121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    15181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    15241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    15301 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    15361 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    15421 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    15481 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    15541 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    15601 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    15661 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    15721 cccagtcacg acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc
    15781 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
    15841 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
    15901 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagaggc gattagtcca
    15961 atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
    16021 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
    16081 aaagggggga a
//
