LOCUS       sequence-99520-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of two gal binding sites coupled wiht a core 
            promoter derived from native Leucine promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS34-Pleum
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(276..296)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(276..294)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(315..331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(315..331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(328..350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    339..355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(363..369,370..387,388..393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    408..429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(546..563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(797..816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaattgggac aacaccagtg aataattctt cacctttaga catttttcta gatagaatgg
       61 tatatccttg aaatatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa
      121 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattggtt
      181 taaaccggag gagagtcttc cgtcggaggg ctgtcgcccg aagcttccgt taattaaggg
      241 gcgcgccaag gatcccggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
      301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      781 aacccgacag gactataaag ataccaggcg tttccccc
//
