LOCUS       sequence-99522-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis 
            with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for 
            crosslinking polyglutamate peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS426-TUB1-internal6xHis - human TUBA1a Cterm...
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             245..262
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgagagaagt tattagtatt aatggtatgt tcgatttgcc cgtccaggct agatcttttt
       61 tttgttagtt cattttggcg tttcatcttt cattactaac ttcaacgcaa aatttttttt
      121 tttttgattt ctctttacag tcggtcaagc tggttgtcag attggtaatg cctgttggga
      181 attatattcc cttgagcacg gtattaagcc ggatggacat ctagaagatg gcctttcaaa
      241 gccgcatcat caccatcatc acaagggagg agaagagggt ttctccacgt ttttccatga
      301 aaccggctac ggtaagttcg ttccaagggc tatttacgtg gatttagagc ccaacgttat
      361 tgacgaagtc cgtaacggtc cttacaagga cttgttccat ccagaacagt tgatcagtgg
      421 taaggaggac gcggctaata attatgcaag aggccattac accgttggta gagaaatttt
      481 gggcgatgtt ctggatagga ttagaaaact ggcagaccaa tcagatgggt tacaagggtt
      541 cttgtttacc cattctcttg gtggtggtac tggttccggt ctagggtcct tactgttgga
      601 agaactatcc gctgaatacg gtaagaaatc caagctggaa tttgccgtat accctgctcc
      661 acaagtgtct acttctgtgg ttgagcctta caacacagtt ttaactacac atactacatt
      721 ggaacatgca gattgtactt tcatggtcga taatgaggct atttacgaca tgtgc
//
