LOCUS       sequence-99523-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of a gal binding site coupled with a core 
            promoter derived from native Leucine promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS1-Pleum
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(274..294)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(274..292)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(313..329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(313..329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(326..348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    337..353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(361..367,368..385,386..391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    406..427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(544..561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccatctaatt caaccaaaat tgggacaaca ccagtgaata attcttcacc tttagacatt
       61 tttctagata gaatggtata tccttgaaat atatatatat atattgctga aatgtaaaag
      121 gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa caataggtcc
      181 ttaaataata ttggtttaaa ccggcggctt ctaatccgaa gcttccgtta attaaggggc
      241 gcgccaagga tcccggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      301 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      361 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      421 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaag
//
