LOCUS       sequence-99524-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of activating sequence derived from native 
            galactose promoter coupled with a core promoter derived from native 
            Leucine promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASGAL-PCYC
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..173
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 aaagctggag ctccgggatc cttggcgcgc cccttaatta acggaagctt tagtacggat
       61 tagaagccgc cgagcgggcg acagccctcc gacggaagac tctcctccgt gcgtcctcgt
      121 cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata
      181 aagattctac aatactagct tttatggtta tgaagaggaa aaattggcag taacctggcc
      241 ccacaaacct tcaaattaac gaatcaaatt aacaaccata ggatgataat gcgattagtt
      301 ttttagcctt atttctgggg taattaatca gcgaagcgat gatttttgat ctattaacag
      361 gtttaaacat ttggcgagcg ttggttggtg gatcaagccc acgcgtaggc aatcctcgag
      421 cagatccgcc aggcgtgtat atatagcgtg gatggccagg caactttagt gctgacacat
      481 acaggcatat atatatgtgt gcgacgacac atgatcatat ggcatgcatg tgctctgtat
      541 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggacc
      601 tttgcagcat aaattactat acttctatag acacgcaaac acaaatacac acactaatct
      661 agaaaaatgt ctaaaggtga agaattattc actggtgttg tcccaatttt ggttgaatta
      721 gatggtgatg ttaatggtca caaattttct gtctccggtg aaggtgaag
//
