LOCUS       sequence-99526-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of two gal binding sites coupled with a core 
            promoter derived from native Leucine promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS12-Pleum
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(272..292)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(272..290)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(311..327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(311..327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(324..346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    335..351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(359..365,366..383,384..389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    404..425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(542..559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaattggg acaacaccag tgaataattc ttcaccttta gacatttttc tagatagaat
       61 ggtatatcct tgaaatatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag
      121 aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgg
      181 tttaaacggc tgtcgccctc ggcggcttct aatccgaagc ttccgttaat taaggggcgc
      241 gccaaggatc ccggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
      301 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      661 caaaaggcca gcaaaaggcc aggaaccgt
//
