LOCUS       sequence-99527-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of activating sequence derived from native 
            galactose promoter coupled with full length native galactose 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASGAL-Pgal
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(70..511)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(76..99)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    394..511
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ccaaaattgg gacaacacca gtgaataatt cttcaccttt agacattttt ctagaatccg
       61 gggttttttc tccttgacgt taaagtatag aggtatatta acaatttttt gttgatactt
      121 ttatgacatt tgaataagaa gtaatacaaa ctgaaaatgt tgaaagtatt agttaaagtg
      181 gttatgcagc ttttccattt atatatctgt taatagatca aaaatcatcg cttcgctgat
      241 taattacccc agaaataagg ctaaaaaact aatcgcatta tcatcctatg gttgttaatt
      301 tgattcgtta atttgaaggt ttgtggggcc aggttactgc caatttttcc tcttcataac
      361 cataaaagct agtattgtag aatctttatt gttcggagca gtgcggcgcg aggcacatct
      421 gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg aggagagtct tccgtcggag
      481 ggctgtcgcc cgctcggcgg cttctaatcc gtactagttt aaacctgtta atagatcaaa
      541 aatcatcgct tcgctgatta attaccccag aaataaggct aaaaaactaa tcgcattatc
      601 atcctatggt tgttaatttg attcgttaat ttgaaggttt gtggggccag gttactgcca
      661 atttttcctc ttcataacca taaaagctag tattgtagaa tctttattgt tcggagcagt
      721 gcggcgcgag gca
//
